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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2015

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Modified chemically defined medium for enhanced respiratory growth of Lactobacillus casei and Lactobacillus plantarum groups

Anno di pubblicazione: 2015

Formato: Elettronico

Autori: Ricciardi A.; Ianniello R.G.; Parente E.; Zotta T.

Affiliazioni autori: Scuola di Scienze Agrarie, Forestali, Alimentari e Ambientali, UniversitÓ degli Studi della Basilicata, Potenza, Italy; Istituto di Scienze dell'Alimentazione-CNR, Avellino, Italy

Autori CNR:

  • TERESA ZOTTA

Lingua: inglese

Abstract: Aims: Members of the Lactobacillus casei and Lactobacillus plantarum groups are capable of aerobic and respiratory growth. However, they grow poorly in aerobiosis in the currently available chemically defined media, suggesting that aerobic and respiratory growth require further supplementation. Methods and Results: The effect of Tween 80, l-alanine, l-asparagine, l-aspartate, l-proline and l-serine on anaerobic and respiratory growth of Lact.ácasei N87 was investigated using a 25 factorial design. The effectiveness of modified CDM (mCDM) was validated on 21 strains of Lact.ácasei and Lact.áplantarum groups. Tween 80 supplementation did not affect anaerobic growth, but improved respiratory growth. l-asparagine, l-proline and l-serine were stimulatory for respiring cells, while the presence of l-aspartate, generally, impaired biomass production. mCDM promoted the growth of Lact.ácasei and Lact.áplantarum, with best results for strains showing a respiratory phenotype. Conclusions: The nutritional requirements of anaerobic and respiratory cultures of members of the Lact.ácasei and Lact.áplantarum groups differ. Tween 80 and selected amino acids derived from pathways related to TCA cycle, pyruvate conversion and NADH recycling are required for respiration. Significance and Impact of the Study: The availability of mCDM will facilitate the study of aerobic metabolism of lactobacilli under controlled conditions.

Lingua abstract: inglese

Pagine da: 776

Pagine a: 785

Rivista:

Journal of applied microbiology Blackwell Science.
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 1365-2672

Numero volume: 119

Numero fascicolo: 3

DOI: 10.1111/jam.12894

Referee: Sý: Internazionale

Stato della pubblicazione: Published version

Indicizzato da: Scopus [2-s2.0-84939494851]

Parole chiave:

  • Aminoacids
  • Chemically defined medium
  • Growth
  • Lactobacillus casei
  • Lactobacillus plantarum
  • Respiration

URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-84939494851&partnerID=q2rCbXpz

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