Home |  English version |  Mappa |  Commenti |  Sondaggio |  Staff |  Contattaci Cerca nel sito  
Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2015

Contributo in rivista

Tipo: Articolo in rivista

Titolo: A new APEH cluster with antioxidant functions in the antarctic hemoglobinless icefish Chionodraco hamatus

Anno di pubblicazione: 2015

Formato: Cartaceo

Autori: Riccio A.; Gogliettino M.; Palmieri G.; Balestrieri M.; Facchiano A.; Rossi M.; Palumbo S.; Monti G.; Cocca E.

Affiliazioni autori: National Research Council, Institute of Biosciences and BioResources (CNR-IBBR), Napoli, Italy; National Research Council, Institute of Food Sciences (CNR-ISA), Avellino, Italy; Ittica Literno Srl, Caserta, Italy

Autori CNR:

  • MARCO BALESTRIERI
  • ENNIO COCCA
  • ANGELO FACCHIANO
  • MARTA GOGLIETTINO
  • GIANNA PALMIERI
  • ALESSIA RICCIO
  • MOSŤ ROSSI

Lingua: inglese

Abstract: Acylpeptide hydrolase (APEH) is a ubiquitous cytosolic protease that plays an important role in the detoxification of oxidised proteins. In this work, to further explore the physiological role of this enzyme, two apeh cDNAs were isolated from the Chionodraco hamatus icefish, which lives in the highly oxygenated Antarctic marine environment. The encoded proteins (APEH-1Ch and APEH-2Ch) were characterised in comparison with the uniquely expressed isoform from the temperate fish Dicentrarchus labrax (APEH-1Dl) and the two APEHs from the red-blooded Antarctic fish Trematomus bernacchii (APEH-1Tb and APEH-2Tb). Homology modelling and kinetic characterisation of the APEH isoforms provided new insights into their structure/function properties. APEH-2 isoforms were the only ones capable of hydrolysing oxidised proteins, with APEH-2Ch being more efficient than APEH-2Tb at this specific function. Therefore, this ability of APEH-2 isoforms is the result of an evolutionary adaptation due to the pressure of a richly oxygenated environment. The lack of expression of APEH-2 in the tissues of the temperate fish used as the controls further supported this hypothesis. In addition, analysis of gene expression showed a significant discrepancy between the levels of transcripts and those of proteins, especially for apeh-2 genes, which suggests the presence of post-transcriptional regulation mechanisms, triggered by cold-induced oxidative stress, that produce high basal levels of APEH-2 mRNA as a reserve that is ready to use in case of the accumulation of oxidised proteins.

Lingua abstract: inglese

Rivista:

PloS one Public Library of Science
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 1932-6203

Numero volume: 10

Numero fascicolo: 5

DOI: 10.1371/journal.pone.0125594

Stato della pubblicazione: Published version

Indicizzato da: Scopus [2-s2.0-84929121474]

Parole chiave:

  • Chionodraco
  • APEH

URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-84929121474&partnerID=q2rCbXpz

Strutture CNR:

Moduli:

 
Torna indietro Richiedi modifiche Invia per email Stampa
Home Il CNR  |  I servizi News |   Eventi | Istituti |  Focus