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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2016

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Adaptive response activated by dietary cis9, trans11 conjugated linoleic acid prevents distinct signs of gliadin-induced enteropathy in mice

Anno di pubblicazione: 2016

Formato: Elettronico Cartaceo

Autori: Bergamo, Paolo; Palmieri, Gianna; Cocca, Ennio; Ferrandino, Ida; Gogliettino, Marta; Monaco, Antonio; Maurano, Francesco; Rossi, Mauro

Affiliazioni autori: Consiglio Nazionale delle Ricerche; Consiglio Nazionale delle Ricerche; Universita degli Studi di Napoli Federico II

Autori CNR:


Lingua: inglese

Abstract: Purpose: The beneficial effects of conjugated linoleic acid (CLA) mixture (cis9, trans11, c9; trans10, cis12, t10) against gliadin-induced toxicity in HLA-DQ8-transgenic mice (DQ8) have been associated with improved duodenal cytoprotective mechanisms [nuclear factor-E2-related factor- 2, Nrf2; acylpeptide hydrolase (APEH)/proteasome]. The present study was aimed at investigating the ability of individual CLA isomers to improve the efficacy of these defensive mechanisms and to protect against duodenal injury caused by the combined administration of gliadin and indomethacin (GI). Methods: Gluten-mediated enteropathy was induced in DQ8 mice by three intra-gastric administration of gliadin (20 mg kg-1/bw) and indomethacin (15 mg L-1) in drinking water for 10 days (GI). C9 or t10 CLA (520 mg kg-1/bw/day) were orally administered for 2 weeks. Pro-oxidant and toxic effects associated with GI treatment, anti-oxidant/detoxifying ability of c9 or t10-CLA and the protective effect induced by c9 pre-treatment (c9 + GI) were evaluated in DQ8 mice duodenum by combining enzymatic, immunoblotting, histological evaluation and quantitative real-time PCR assays. Results: GI treatment produces the time-dependent decline of the considered detoxifying mechanisms thus leading to pro-apoptotic and pro-oxidant effects. APEH/proteasome pathway was not markedly affected by individual CLA isomers, but duodenal redox status and activity/mRNA levels of Nrf2-activated enzymes were significantly improved by c9 administration. c9 pre-treatment protects against GI-mediated accumulation of oxidative stress markers, and histological examination reveals the increase of goblet cells number in mouse duodenum but induces only a partial recovery of APEH/proteasome activity. Conclusions: The activation of and adaptive response by low doses of c9 supplementation prevents distinct signs of gliadin-induced enteropathy in DQ8 mice

Lingua abstract: inglese


European journal of nutrition Steinkopff.
Paese di pubblicazione: Germania
Lingua: inglese
ISSN: 1436-6207

DOI: 10.1007/s00394-015-0893-2

Referee: Sė: Internazionale

Stato della pubblicazione: Published version

Indicizzato da: Scopus [2-s2.0-84926392830]

Parole chiave:

  • Adaptive response
  • Celiac disease
  • Conjugated linoleic acid
  • Fatty acids
  • Gliadin
  • Nrf2

URL: http://www.scopus.com/record/display.url?eid=2-s2.0-84926392830&origin=inward

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