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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2014

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Ovothiol Isolated from Sea Urchin Oocytes Induces Autophagy in the Hep-G2 Cell Line

Anno di pubblicazione: 2014

Formato: Elettronico

Autori: Russo, Gian Luigi; Russo, Maria; Castellano, Immacolata; Napolitano, Alessandra; Palumbo, Anna

Affiliazioni autori: Consiglio Nazionale delle Ricerche (CNR); Stn Zool Anton Dohrn; University of Naples Federico II

Autori CNR:

  • GIAN LUIGI RUSSO
  • MARIA RUSSO

Lingua: inglese

Abstract: Ovothiols are histidine-derived thiols isolated from sea urchin eggs, where they play a key role in the protection of cells toward the oxidative burst associated with fertilization by controlling the cellular redox balance and recycling oxidized glutathione. In this study, we show that treatment of a human liver carcinoma cell line, Hep-G2, with ovothiol A, isolated from Paracentrotus lividus oocytes, results in a decrease of cell proliferation in a dose-dependent manner. The activation of an autophagic process is revealed by phase contrast and fluorescence microscopy, together with the expression of the specific autophagic molecular markers, LC3 II and Beclin-1. The effect of ovothiol is not due to its antioxidant capacity or to hydrogen peroxide generation. The concentration of ovothiol A in the culture media, as monitored by HPLC analysis, decreased by about 24% within 30 min from treatment. The proliferation of normal human embryonic lung cells is not affected by ovothiol A. These results hint at ovothiol as a promising bioactive molecule from marine organisms able to inhibit cell proliferation in cancer cells.

Lingua abstract: inglese

Pagine da: 4069

Pagine a: 4085

Pagine totali: 17

Rivista:

Marine drugs MDPI,
Paese di pubblicazione:
Lingua: inglese
ISSN: 1660-3397

Numero volume: 12

Numero fascicolo: 7

DOI: 10.3390/md12074069

Referee: Sì: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000339989300017]

Parole chiave:

  • ovothiols
  • autophagy
  • sea urchin
  • Hep-G2

Strutture CNR:

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