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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2014

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Inhibition of protein kinase CK2 by quercetin enhances CD95-mediated apoptosis in a human thymus-derived T cell line

Anno di pubblicazione: 2014

Formato: Cartaceo

Autori: Russo, Maria; Spagnuolo, Carmela; Bilotto, Stefania; Tedesco, Idolo; Maiani, Giuseppe; Russo, Gian Luigi

Affiliazioni autori: Consiglio Nazionale delle Ricerche (CNR); Res Ctr Food & Nutr CRA NUT

Autori CNR:


Lingua: inglese

Abstract: Quercetin (3,3',4',5,7-pentahydroxyflavone) is one of the major dietary flavonoid, found in a broad range of fruits, vegetables and beverages, such as tea and wine, with a daily uptake between 10 and 100 mg depending on the dietary attitude. We previously demonstrated that quercetin sensitizes HPB-ALL cell line to CD95-mediated apoptosis through a mechanism independent of its antioxidant properties and related to its ability to regulate the activity of serine-threonine protein kinases. Here, we report that quercetin is an inhibitor of protein kinase CK2, a kinase involved in apoptotic resistance in several forms of leukemia. Quercetin inhibits CK2 in an in vitro assay and in HPB-ALL cells decreasing its activity of 3.5-fold within 12 h from treatment, in good agreement with the intracellular concentration of the molecule measured by HPLC on the same cells (0.933 +/- 0.188 mu g/mg of total proteins). Quercetin also inhibits pure CK2 enzyme with an EC50 of about 150 nM in an in vitro assay. A specific CK2 inhibitor, TBB (4,5,6,7-tetra-bromo-benzo-triazole), behaves similarly to quercetin since it abolishes CK2 activity in HPB-ALL cells, but fails to enhance CD95-mediated apoptosis when associated with an anti-CD95 monoclonal antibody mimicking CD95 ligand. The resistance to apoptotic induction in HPB-ALL can be bypassed taking advantage of the pleiotropic activity of quercetin. Combined treatments which include quercetin in association with death receptor inducers may represent a novel therapeutic strategy against leukemia. (C) 2014 Elsevier Ltd. All rights reserved.

Lingua abstract: inglese

Pagine da: 244

Pagine a: 251

Pagine totali: 8


Food research international Elsevier Applied Science on behalf of the Canadian Institute of Food Science and Technology,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0963-9969

Numero volume: 63

DOI: 10.1016/j.foodres.2014.05.022

Referee: Sė: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000342552300016]

Parole chiave:

  • Quercetin
  • Protein kinase CK2
  • Apoptosis
  • CD95/Fas
  • Polyphenol
  • Flavonoids

Strutture CNR:


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