Home |  English version |  Mappa |  Commenti |  Sondaggio |  Staff |  Contattaci Cerca nel sito  
Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2013

Contributo in rivista

Tipo: Articolo in rivista

Titolo: T-Type Ca2+Current Activity during Oocyte Growth and Maturation in the Ascidian Styela plicata

Anno di pubblicazione: 2013

Formato: Elettronico

Autori: Gallo, Alessandra; Russo, Gian Luigi; Tosti, Elisabetta

Affiliazioni autori: Stn Zool Anton Dohrn; Consiglio Nazionale delle Ricerche (CNR)

Autori CNR:

  • GIAN LUIGI RUSSO

Abstract: Voltage-dependent calcium currents play a fundamental role during oocyte maturation, mostly L-type calcium currents, whereas T-type calcium currents are involved in sperm physiology and cell growth. In this paper, using an electrophysiological and pharmacological approach, we demonstrated, for the first time in oocytes, that T-type calcium currents are present with functional consequences on the plasma membrane of growing immature oocytes of the ascidian Styela plicata. We classified three subtypes of immature oocytes at the germinal vesicle stage on the basis of their size, morphology and accessory cellular structures. These stages were clearly associated with an increased activity of T-type calcium currents and hyperpolarization of the plasma membrane. We also observed that T-type calcium currents oscillate in the post-fertilization embryonic stages, with minimal amplitude of the currents in the zygote and maximal at 8-cell stage. In addition, chemical inhibition of T-type calcium currents, obtained by applying specific antagonists, induced a significant reduction in the rate of cleavage and absence of larval formation. We suggest that calcium entry via T-type calcium channels may act as a potential pacemaker in regulating cytosolic calcium involved in fertilization and early developmental events.

Lingua abstract: inglese

Pagine da: e54604

Pagine totali: 11

Rivista:

PloS one Public Library of Science
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 1932-6203

Numero volume: 8

Numero fascicolo: 1

DOI: 10.1371/journal.pone.0054604

Referee: Sì: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000314019100083]

Strutture CNR:

Moduli:

Allegati: T-Type Ca2+ Current Activity during Oocyte Growth and Maturation in the Ascidian Styela plicata (application/pdf)

 
Torna indietro Richiedi modifiche Invia per email Stampa
Home Il CNR  |  I servizi News |   Eventi | Istituti |  Focus