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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2015

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Polymorphisms in stress response genes in Lactobacillus plantarum: implications for classification and heat stress response

Anno di pubblicazione: 2015

Formato: Elettronico

Autori: Guidone, Angela; Parente, Eugenio; Zotta, Teresa; Guinane, Caitriona M.; Rea, Mary C.; Stanton, Catherine S.; Ross, Reynolds Paul; Ricciardi, Annamaria

Affiliazioni autori: Universita degli Studi della Basilicata; Istituto di Scienze dell'Alimentazione; Teagasc - Irish Agriculture and Food Development Authority; University College Cork

Autori CNR:

  • EUGENIO PARENTE
  • TERESA ZOTTA

Lingua: inglese

Abstract: The polymorphism of 5 stress response genes (hrcA, ctsR, clpP, ftsH, dnaK) in 32 Lactobacillus plantarum strains was evaluated by multilocus restriction typing (MLRT) and by sequence analysis of ctsR, hrcA and clpP genes. Both these approaches allowed the discrimination of the subspecies L. plantarum ssp. plantarum and L. plantarum ssp. argentoratensis. HrcA sequence analysis also allowed discrimination at the species and subspecies level of several species of lactic acid bacteria, thus confirming that it can be used as a valuable taxonomic marker. No significant relationship was found between stress response gene polymorphism and resistance to heat treatments. The effect of temperature on growth kinetics and the protein expression were investigated for selected strains carrying different mutations in hrcA. L. plantarum ssp. argentoratensis NCIMB12120 and L. plantarum ssp. plantarum DPC2159, both of which had mutations in domains of HrcA which are important for the repressor functionality, had a reduced growth rate at all temperatures tested (25, 30, 37, 40, and 42 C) compared to L. plantarum WCFS1. In L. plantarum DPC2159, protein expression upon temperature shifts from 25 to 40 C or growth at 40 C was altered compared to L. plantarum WCFS1, but further study is needed to unequivocally confirm the relationship with mutations in hrcA.

Lingua abstract: inglese

Pagine da: 297

Pagine a: 305

Rivista:

Annals of microbiology Springer
Paese di pubblicazione: Germania
Lingua: inglese
ISSN: 1590-4261

Numero volume: 65

DOI: 10.1007/s13213-014-0862-7

Referee: S: Internazionale

Stato della pubblicazione: Published version

Indicizzato da: Scopus [2-s2.0-84896690907]

Parole chiave:

  • Heat stress
  • Lactobacillus plantarum
  • Polymorphism
  • Stress response genes

URL: http://www.scopus.com/record/display.url?eid=2-s2.0-84896690907&origin=inward

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