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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2015

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Metabolic profiling and stress response of anaerobic and respiratory cultures of Lactobacillus plantarum C17 grown in a chemically defined medium

Anno di pubblicazione: 2015

Formato: Elettronico

Autori: Ricciardi, Annamaria; Castiglione Morelli, Maria Antonietta; Ianniello, Rocco Gerardo; Parente, Eugenio; Zotta, Teresa

Affiliazioni autori: Universita degli Studi della Basilicata; Istituto di Scienze dell'Alimentazione

Autori CNR:

  • EUGENIO PARENTE
  • TERESA ZOTTA

Lingua: inglese

Abstract: Aims: Members of the Lactobacillus casei and Lactobacillus plantarum groups are capable of aerobic and respiratory growth. However, they grow poorly in aerobiosis in the currently available chemically defined media, suggesting that aerobic and respiratory growth require further supplementation. Methods and Results: The effect of Tween 80, L-alanine, L-asparagine, L-aspartate, L-proline and L-serine on anaerobic and respiratory growth of Lact. casei N87 was investigated using a 25 factorial design. The effectiveness of modified CDM (mCDM) was validated on 21 strains of Lact. casei and Lact. plantarum groups. Tween 80 supplementation did not affect anaerobic growth, but improved respiratory growth. L-asparagine, L-proline and L-serine were stimulatory for respiring cells, while the presence of L-aspartate, generally, impaired biomass production. mCDM promoted the growth of Lact. casei and Lact. plantarum, with best results for strains showing a respiratory phenotype. Conclusions: The nutritional requirements of anaerobic and respiratory cultures of members of the Lact. casei and Lact. plantarum groups differ. Tween 80 and selected amino acids derived from pathways related to TCA cycle, pyruvate conversion and NADH recycling are required for respiration. Significance and Impact of the Study: The availability of mCDM will facilitate the study of aerobic metabolism of lactobacilli under controlled conditions.

Lingua abstract: inglese

Pagine da: 1639

Pagine a: 1648

Rivista:

Annals of microbiology Springer
Paese di pubblicazione: Germania
Lingua: inglese
ISSN: 1590-4261

Numero volume: 65

DOI: 10.1007/s13213-014-1003-z

Referee: Sė: Internazionale

Stato della pubblicazione: Published version

Indicizzato da: Scopus [2-s2.0-84912544333]

Parole chiave:

  • Chemically defined medium
  • Lactobacillus plantarum
  • Metabolic profile
  • Respiration

URL: http://www.scopus.com/record/display.url?eid=2-s2.0-84912544333&origin=inward

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