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Contributo in rivista
Tipo: Articolo in rivista
Titolo: A signature protein-based method to distinguish Mediterranean water buffalo and foreign breed milk
Anno di pubblicazione: 2013
Autori: Caira S.; Pinto G.; Balteanu V.A.; Chianese L.; Addeo F.
Affiliazioni autori: UniversitÓ Degli Studi di Napoli Federico II, FacoltÓ di Agraria, Via UniversitÓ, 100, 80055 Portici, Napoli, Italy
Abstract: A novel genetic variant at the ?s1-casein locus of water buffalo (WB), 8-residue shorter than its wild-type has been found and sequenced. The internal deletion of the peptide E(35)KVNELsT(42) was confirmed by the isolation of the junction peptide. The 8-residue deletion mutant has a molecular weight that is 919 Da less than that of the wild-type. The novel isoform with a unique f35-42 deletion could be the result of the skipping of exon 6, generating an exon 6-deleted variant of ?s1-casein. The wild-type and its shortened ?s1-casein forms were found to co-exist in many individual milk samples. In contrast, the 8-residue, internally deleted ?s1-casein variant did not occur in water buffaloes of the Mediterranean breed reared in Italy. Wild-type ?s1-casein has 6 to 8 phosphate groups (P) while the internally deleted form 6 and 7P per molecule.
Lingua abstract: inglese
Pagine da: 597
Pagine a: 603
Applied Science Publishers.
Numero volume: 141
Numero fascicolo: 1
Indicizzato da: Scopus [2-s2.0-84877800449]
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