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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2015

Contributo in rivista

Tipo: Articolo in rivista

Titolo: High resolution melting analysis (HRM) as a new tool for the identification of species belonging to the Lactobacillus casei group and comparison with species-specific PCRs and multiplex PCR

Anno di pubblicazione: 2015

Formato: Elettronico

Autori: Iacumin, Lucilla; Ginaldi, Federica; Manzano, Marisa; Anastasi, Veronica; Reale, Anna; Zotta, Teresa; Rossi, Franca; Coppola, Raffaele; Comi, Giuseppe

Affiliazioni autori: University of Udine; Istituto di Scienze dell'Alimentazione del Consiglio Nazionale delle Ricerche (CNR); University of Molise

Autori CNR:

  • ANNA REALE
  • TERESA ZOTTA

Lingua: inglese

Abstract: The correct identification and characterisation of bacteria is essential for several reasons: the classification of lactic acid bacteria (LAB) has changed significantly over the years, and it is important to distinguish and define them correctly, according to the current nomenclature, avoiding problems in the interpretation of literature, as well as mislabelling when probiotic are used in food products. In this study, species-specific PCR and HRM (high-resolution melting) analysis were developed to identify strains belonging to the Lactobacillus casei group and to classify them into L. casei, Lactobacillus paracasei and Lactobacillus rhamnosus. HRM analysis confirmed to be a potent, simple, fast and economic tool for microbial identification.

Lingua abstract: inglese

Altro abstract: In particular, 201 strains, collected from International collections and attributed to the L. casei group, were examined using these techniques and the results were compared with consolidated molecular methods, already published. Seven of the tested strains don't belong to the L. casei group. Among the remaining 194 strains, 6 showed inconsistent results, leaving identification undetermined. All the applied techniques were congruent for the identification of the vast majority of the tested strains (188). Notably, for 46 of the strains, the identification differed from the previous attribution. (C) 2014 Elsevier Ltd. All rights reserved.

Pagine da: 357

Pagine a: 367

Pagine totali: 11

Rivista:

Food microbiology Academic Press,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0740-0020

Numero volume: 46

DOI: 10.1016/j.fm.2014.08.007

Referee: Sė: Internazionale

Stato della pubblicazione: Published version

Indicizzato da: ISI Web of Science (WOS) [000345992200047]

Parole chiave:

  • Lactobacillus casei group
  • High resolution melting analysis
  • Identification methods
  • Multiplex PCR
  • Species-specific PCR

Strutture CNR:

 
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