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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2014

Contributo in rivista

Tipo: Articolo in rivista

Titolo: The E1 beta-subunit of pyruvate dehydrogenase is surface-expressed in Lactobacillus plantarum and binds fibronectin

Anno di pubblicazione: 2014

Formato: Elettronico

Autori: Vastano V.; Salzillo M.; Siciliano R.A.; Muscariello L.; Sacco M.; Marasco R.

Affiliazioni autori: Dipartimento di Scienze e Tecnologie Ambientali, Biologiche, e Farmaceutiche, Seconda Università di Napoli, via Vivaldi 43, 81100 Caserta, Italy; Centro di Spettrometria di Massa Proteomica e Biomolecolare, Istituto di Scienze dell'Alimentazione, CNR, Avellino, Italy

Autori CNR:

  • ROSA ANNA SICILIANO

Lingua: inglese

Abstract: Lactobacillus plantarum is among the species with a probiotic activity. Adhesion of probiotic bacteria to host tissues is an important principle for strain selection, because it represents a crucial step in the colonization process of either pathogens or commensals. Most bacterial adhesins are proteins, and a major target for them is fibronectin, an extracellular matrix glycoprotein. In this study we demonstrate that PDHB, a component of the pyruvate dehydrogenase complex, is a factor contributing to fibronectin-binding in L. plantarum LM3. By means of fibronectin overlay immunoblotting assay, we identified a L. plantarum LM3 surface protein with apparent molecular mass of 35. kDa. Mass spectrometric analysis shows that this protein is the pyruvate dehydrogenase E1 beta-subunit (PDHB). The corresponding pdhB gene is located in a 4-gene cluster encoding pyruvate dehydrogenase. In LM3-B1, carrying a null mutation in pdhB, the 35. kDa adhesin was not anymore detectable by immunoblotting assay. Nevertheless, the pdhB null mutation did not abolish pdhA, pdhC, and pdhD transcription in LM3-B1. By adhesion assays, we show that LM3-B1 cells bind to immobilized fibronectin less efficiently than wild type cells. Moreover, we show that pdhB expression is negatively regulated by the CcpA protein and is induced by bile. © 2013.

Lingua abstract: inglese

Pagine da: 121

Pagine a: 127

Rivista:

Microbiological research Elsevier.
Paese di pubblicazione: Germania
Lingua: inglese
ISSN: 0944-5013

Numero volume: 169

Numero fascicolo: 2-3

DOI: 10.1016/j.micres.2013.07.013

Referee: Sì: Internazionale

Indicizzato da: Scopus [2-s2.0-84891631049]

Parole chiave:

  • Adhesins
  • Fibronectin
  • Lactobacillus plantarum
  • PDHB
  • Probiotics

URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-84891631049&partnerID=q2rCbXpz

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