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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2014

Contributo in rivista

Tipo: Articolo in rivista

Titolo: In vitro study of AFB(1) and AFM(1) effects on human lymphoblastoid Jurkat T-cell model

Anno di pubblicazione: 2014

Autori: Luongo, D.; Russo, R.; Balestrieri, A.; Marzocco, S.; Bergamo, P.; Severino, L.

Affiliazioni autori: University of Naples Federico II; NRC; University of Salerno

Autori CNR:

  • PAOLO BERGAMO
  • DIOMIRA LUONGO

Abstract: Aflatoxin B-1 (AFB(1)) is a mycotoxin produced by Aspergillus spp. that can occur as a natural contaminant in foods and feeds of vegetable origin. Post-ingestion, AFB(1) can be metabolized in the liver of mammals into hydroxylated aflatoxin M-1 (AFM(1)) that is excreted with milk. Although several studies have been carried out to evaluate effects of AFB(1) on the immune system, studies regarding AFM(1) are moreover lacking. The aim of the current study was to investigate effects of AFB(1) and AFM(1) on immune function using a lymphoblastoid Jurkat T-cell line as an experimental model. Both AFB(1) and AFM(1) produced significant decreases in Jurkat cell proliferation, whereas only minor effects were noted on interleukin (IL)-2 and interferon (IFN)-gamma cytokines mRNA expression in stimulated cells that had been pre-incubated with AFB(1) and AFM(1). Particularly, AFB(1), but not AFM(1), at the highest concentration (50 mu M) induced a marked increase in IL-8 mRNA expression. The results of the current study suggested the existence of a concentration threshold for AFB(1) and AFM(1) needed to exert biological activity on cell viability and innate immunity.

Lingua abstract: inglese

Pagine da: 353

Pagine a: 358

Pagine totali: 6

Rivista:

Journal of immunotoxicology Taylor & Francis Health Sciences,
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 1547-691X

Numero volume: 11

Numero fascicolo: 4

DOI: 10.3109/1547691X.2013.848250

Indicizzato da: ISI Web of Science (WOS) [000343720800008]

Parole chiave:

  • Aflatoxin B-1
  • aflatoxin M-1
  • Jurkat cells

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