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Torna all'elenco Contributi in rivista anno 2014

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Fractionation of complex lipid mixtures by hydroxyapatite chromatography for lipidomic purposes

Anno di pubblicazione: 2014

Formato: Elettronico Cartaceo

Autori: Pinto, Gabriella; Caira, Simonetta; Mamone, Gianfranco; Ferranti, Pasquale; Addeo, Francesco; Picariello, Gianluca

Affiliazioni autori: University of Naples Federico II; Consiglio Nazionale delle Ricerche (CNR)

Autori CNR:

  • SIMONETTA CAIRA
  • GIANFRANCO MAMONE
  • GIANLUCA PICARIELLO

Lingua: inglese

Abstract: The comprehensive analysis of natural lipid mixtures is often challenging due to the simultaneous occurrence of functionally and structurally heterogeneous compounds. Modern analytical approaches in system-wide lipidomics essentially rely on mass spectrometry (MS). The overabundant amount of triacylglycerols (TAGs) in most samples hinders the direct detection of phospholipids (PLs) or other low-abundance lipids; therefore, a fractionation step is most often required to reduce sample complexity prior to MS analysis. In this work, we explore the use of hydroxyapatite (HAP) as a chromatographic stationary phase (gel HAP) or chemo-affinity sorbent material (ceramic HAP) to selectively enrich PLs and polar lipids from chicken egg yolk and buttermilk. Due to the affinity of phosphate-containing compounds for HAP, both in-column and in-batch HAP-based chromatography were effective to deplete TAGs before releasing PLs with an eluting ternary system containing sodium phosphate as the displacing agent. Buttermilk gangliosides and PLs co-eluted, indicating that HAP also exhibits a high affinity for sialylated glycolipids and that polar lipids are retained through a combined mechanism of chemo-affinity and hydrogen bonding. To the best of our knowledge, this is the first report in which HAP is proposed as an alternative stationary phase for separating both the PLs and sialylated glycolipids from TAGs in complex lipidomes. The HAP-based chromatography has potential to be improved for the separation of the polar lipid classes. (C) 2014 Elsevier B.V. All rights reserved.

Lingua abstract: inglese

Pagine da: 82

Pagine a: 92

Pagine totali: 11

Rivista:

Journal of chromatography Elsevier
Paese di pubblicazione: Paesi Bassi
Lingua: inglese
ISSN: 0021-9673

Numero volume: 1360

DOI: 10.1016/j.chroma.2014.07.093

Referee: Sė: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000341469400010]

Parole chiave:

  • Phospholipids
  • Hydroxyapatite chromatography
  • Egg yolk
  • Buttermilk
  • Gangliosides
  • Lipidomics

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