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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2000

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Immunochemical evaluation of bovine beta-casein and its 1-28 phosphopeptide in cheese during ripening

Anno di pubblicazione: 2000

Formato: Elettronico Cartaceo

Autori: Pizzano, R; Nicolai, MA; Padovano, P; Ferranti, P; Barone, F; Addeo, F

Affiliazioni autori: Consiglio Nazionale delle Ricerche (CNR); University of Naples Federico II

Autori CNR:

  • ROSA PIZZANO

Lingua: inglese

Abstract: Polyclonal antibodies raised against the plasmin-released 1-28 phosphopeptide from bovine beta -casein [i.e., beta -CN(f1-28)4P] specifically recognized the tryptic beta -casein 1-25 and 2-25 peptides, whatever the degree of phosphorylation, but were unresponsive to the shortened beta -casein 16-22 phosphopeptide. These antibodies were able to recognize the parent bovine beta -casein as well as the homologous water buffalo protein, but they could not detect the homologous counterparts from ovine and caprine milks. Such antibodies were used in competitive enzyme-linked immunosorbent assays to monitor the plasmin-mediated release of the 1-28 phosphopeptide from beta -casein and to evaluate the residual native beta -casein in bovine cheese sampled during ripening. Applications of these polyclonal antibodies are suggested mainly for estimating the age of hard cheeses and, possibly, for tracing the presence of bovine casein in fresh ovine and caprine cheeses.

Lingua abstract: inglese

Pagine da: 4555

Pagine a: 4560

Pagine totali: 6

Rivista:

Journal of agricultural and food chemistry American Chemical Society, Books and Journals Division]
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0021-8561

Numero volume: 48

Numero fascicolo: 10

DOI: 10.1021/jf000498o

Referee: Sė: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000089957200017]

Parole chiave:

  • cheese
  • ripening
  • quality certification
  • phosphopeptides
  • antipeptide antibodies
  • ELISA
  • immunoblotting

Strutture CNR:

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