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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2014

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Isoelectric Focusing and ELISA for Detecting Adulteration of Donkey Milk with Cow Milk

Anno di pubblicazione: 2014

Formato: Elettronico Cartaceo

Autori: Pizzano Rosa; Salimei Elisabetta

Affiliazioni autori: Consiglio Nazionale delle Ricerche (CNR); Università degli Studi del Molise

Autori CNR:

  • ROSA PIZZANO

Lingua: inglese

Abstract: Donkey milk has been recently revalued intensely due to its nutritional properties. Moreover, donkey milk has been proposed as an effective alternative food for some infants with cow milk allergy. Two fast analytical methods were proposed to detect the fraudulent practice of blending cow milk to donkey milk. Detection of cow a alpha(s1)-casein bands along the profiles of experimental donkey cow milk mixtures analyzed by isoelectric focusing was adequate to estimate cow milk used as adulterant of donkey milk starting from 5% (v/v). An ELISA-based method using the antipeptide antibodies raised against the 1-28 sequence stretch of cow beta-casein was also developed for an accurate definition of composition of donkey cow milk mixtures. The presence of cow milk at levels as low as 0.5% (v/v) was detected in donkey cow milk mixtures prepared at laboratory scale and assayed by ELISA.

Lingua abstract: inglese

Pagine da: 5853

Pagine a: 5858

Pagine totali: 6

Rivista:

Journal of agricultural and food chemistry American Chemical Society, Books and Journals Division]
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0021-8561

Numero volume: 62

Numero fascicolo: 25

DOI: 10.1021/jf5025533

Referee: Sì: Internazionale

Stato della pubblicazione: Published version

Indicizzato da:

  • ISI Web of Science (WOS) [000338183900018]
  • Scopus [2-s2.0-84903281035]
  • PubMed [24892189]

Parole chiave:

  • donkey milk
  • adulteration
  • antipeptide antibodies
  • cow milk allergy
  • PAGIF
  • immunoblotting
  • ELISA

Strutture CNR:

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