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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2014

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Isolation and functional characterization of a novel gene coding for flavonoid 3'-hydroxylase from globe artichoke

Anno di pubblicazione: 2014

Formato: Elettronico

Autori: DE PALMA M., FRATIANNI F., NAZZARO F., and TUCCI M

Affiliazioni autori: De Palma M.- IBBR Institute of Biosciences and Bioresources, CNR, I-80055, Portici, Italy Fratianni F. -Institute of Food Science and Technology, CNR, I-83100, Avellino, Italy Nazzaro F. - Institute of Food Science and Technology, CNR, I-83100, Avellino, Italy Tucci M. - IBBR Institute of Biosciences and Bioresources, CNR, I-80055, Portici, Italy

Autori CNR:

  • MONICA DE PALMA
  • FLORINDA FRATIANNI
  • FILOMENA NAZZARO
  • MARINA TUCCI

Lingua: inglese

Abstract: Globe artichoke (Cynara cardunculus L. var. scolymus) is rich in flavonoids which contribute to its health-promoting properties. With the aim of understanding the genetic control of flavonoid accumulation in artichoke, we isolated an artichoke full-length cDNA sequence encoding flavonoid 3'-hydroxylase (F3'H), a major enzyme of the flavonoid hydroxylation pattern. In silico studies confirmed that the deduced amino acid sequence of CcF3'H is highly similar to F3'Hs isolated from other Asteraceae. The Northern blot analysis demonstrated that CcF3'H was highly expressed in leaves and in specific parts of the heads. Its expression differed slightly among artichoke cultivars. The overexpression of CcF3'H in tobacco plants led to the accumulation of flavonoids and to an increase of flower colour intensity, thus identifying CcF3'H as promising candidate for genetic engineering. CcF3'H represents the first structural gene of the flavonoid biosynthesis isolated from C. cardunculus, and its characterization sheds light on the accumulation of flavonoids.

Lingua abstract: inglese

Pagine da: 445

Pagine a: 455

Pagine totali: 10

Rivista:

Biologia plantarum Academia
Paese di pubblicazione: Repubblica Ceca
Lingua: inglese
ISSN: 0006-3134

Numero volume: 58

Numero fascicolo: 3

DOI: 10.1007/s10535-014-0424-7

Referee: Sì: Internazionale

Stato della pubblicazione: Published version

Parole chiave:

  • anthocyanins
  • Cynara cardunculus
  • expression analysis
  • flavonoid biosynthesis
  • transgenic plants

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