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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2014

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Tolerogenic effect of mesenchymal stromal cells on gliadin-specific T lymphocytes in celiac disease

Anno di pubblicazione: 2014

Formato: Elettronico Cartaceo

Autori: Ciccocioppo R1, Camarca A2, Cangemi GC3, Radano G2, Vitale S2, Betti E3, Ferrari D4, Visai L5, Strada E6, Badulli C7, Locatelli F8, Klersy C9, Gianfrani C2, Corazza GR3.

Affiliazioni autori: 1Clinica Medica I, Fondazione IRCCS Policlinico San Matteo, UniversitÓ di Pavia, Pavia, Italy. Electronic address: rachele.ciccocioppo@unipv.it. 2Istituto Scienze dell'Alimentazione, CNR Avellino, Italy. 3Clinica Medica I, Fondazione IRCCS Policlinico San Matteo, UniversitÓ di Pavia, Pavia, Italy. 4Dipartimento di Bioscienze, UniversitÓ di Parma, Italy (currently affiliated with Dipartimento di Biochimica e Biologia Molecolare, UniversitÓ di Parma, Italy; Dipartimento di Medicina Occupazionale, Ergonomia e DisabilitÓ, Laboratorio di Nanotecnologia, Fondazione IRCCS Salvatore Maugeri, UniversitÓ di Pavia, Italy). 5Laboratorio di Nanotecnologia, Fondazione IRCCS Salvatore Maugeri, UniversitÓ di Pavia, Italy (currently affiliated with Dipartimento di Medicina Occupazionale, Ergonomia e DisabilitÓ, Laboratorio di Nanotecnologia, Fondazione IRCCS Salvatore Maugeri, UniversitÓ di Pavia, Italy; Dipartimento di Medicina Molecolare, Centro di Ingegneria Tissutale, INSTM UdR Pavia, UniversitÓ di Pavia, Italy). 6Servizio di Endoscopia Digestiva, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. 7Servizio di Immunogenetica, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. 8Dipartimento di Onco-Ematologia Pediatrica e Medicina Trasfusionale, Ospedale Bambino Ges¨, Roma e UniversitÓ degli Studi di Pavia, Pavia, Italy. 9Servizio di Biometria e Statistica, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Autori CNR:

  • ALESSANDRA CAMARCA
  • CARMELA GIANFRANI

Lingua: inglese

Abstract: Background aims. Celiac disease is caused by a dysregulated immune response toward dietary gluten, whose only treatment is a lifelong gluten-free diet. We investigated the effects of mesenchymal stromal cells (MSCs) on gliadin-specific T cells, which are known to induce intestinal lesions, in view of a possible use as new therapy. Methods. Bone marrow derived MSCs and gliadin-specific T-cell lines were obtained from allogeneic donors and mucosal specimens of celiac patients, respectively. The immunosuppressant effect of MSCs was evaluated in terms of proliferative response and interferon (IFN)-gamma production upon gliadin stimulation of long-term T-cell lines; the immunomodulant effect was assessed in terms of apoptotic rate, immunophenotype and cytokine profile of short-term T-cell lines generated in the presence of MSCs. Different MSC:T-cell ratios were applied, and statistics were performed as appropriate. Results. MSCs inhibited both proliferative response and IFN-gamma production of long-term T-cell lines in a dose-dependent manner while limiting the expansion of short-term T-cell lines by increasing the apoptotic rate. Moreover, a reduction of the CD4(+) population and expansion of the regulatory FoxP3(+) subset were found in T-cell lines cultured with MSCs, in which a significant decrease of interleukin (IL)-21, IFN-gamma and IL-10 paralleled by an upregulation of transforming growth factor-beta 1, IL-6 and IL-8 were observed. Finally, an increase of the indoleamine 2,3-dioxygenase activity was found, possibly playing a key role in mediating these effects. Conclusions. MSCs exert potent immunomodulant effects on gliadin-specific T cells, which may be exploited for future therapeutic application in celiac disease.

Lingua abstract: inglese

Pagine da: 1080

Pagine a: 1091

Pagine totali: 12

Rivista:

Cytotherapy Informa Healthcare
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 1465-3249

Numero volume: 16

Numero fascicolo: 8

DOI: 10.1016/j.jcyt.2014.03.002

Referee: Sý: Internazionale

Stato della pubblicazione: Published version

Indicizzato da: ISI Web of Science (WOS) [000338819200006]

Parole chiave:

  • celiac disease
  • immune tolerance
  • mesenchymal stromal cells
  • T lymphocytes

Strutture CNR:

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