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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2014

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Mass spectrometry analysis of in vitro Nuclear Aggregates of Polyamines

Anno di pubblicazione: 2014

Formato: Elettronico Cartaceo

Autori: Picariello Gianluca; Iacomino, Giuseppe; (*) Di Luccia Aldo; D'Agostino Luciano;

Affiliazioni autori: Institute of Food Science - CNR; (*) University of Foggia, Dpt. of Agronomy, Food and Environment (SAFE)

Autori CNR:

  • LUCIANO D'AGOSTINO
  • ALDO DI LUCCIA
  • GIUSEPPE IACOMINO
  • GIANLUCA PICARIELLO

Lingua: inglese

Abstract: RATIONALE: In the nuclei of eukaryotic cells, polyamines and phosphate ions self-assemble via ionic interactions and hydrogen bonding, generating three families of supramolecular compounds that have been named large (l-), medium (m-) and small (s-) nuclear aggregates of polyamines (NAPs). In a simulated nuclear environment, polyamines and phosphate ions generate the in vitro NAPs (ivNAPs) that share strict structural and functional analogies with their cellular cognates. Mass spectrometric data are expected to provide important structural details of NAPs/ivNAPs. METHODS: We used both electrospray (ESI) and nitrocellulose (NC) matrix assisted laser desorption ionization - time of flight (MALDI-TOF) mass spectrometry (MS) to support a variety of analytical techniques previously addressed to structurally characterize NAPs/ivNAPs. RESULTS: The dominant m/z values of s-ivNAP (m/z 735, 749, 761) are compatible with a defined set of cyclic or linear aggregates. On the basis of the experimental molecular mass (a cluster centred at m/z 2980), the m-ivNAP corresponds to the supramolecular assembly of 4 modules of s-ivNAPs. No informative mass spectra were obtained for the l-ivNAP. CONCLUSIONS: MS data support the models of NAPs that have been inferred by using an array of analytical techniques. NC MALDI-MS contributed much more effectively than ESI-MS to the structural characterization of ivNAPs.

Lingua abstract: inglese

Pagine da: 499

Pagine a: 504

Pagine totali: 6

Rivista:

Rapid communications in mass spectrometry John Wiley & Sons
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 1097-0231

Numero volume: 28

Numero fascicolo: 5

DOI: 10.1002/rcm.6807

Referee: Sì: Internazionale

Indicizzato da: PubMed [24497288]

Parole chiave:

  • Nuclear Aggregates of Polyamines; supramolecular self-assembly; ESI MS; MALDI-TOF MS.

URL: http://onlinelibrary.wiley.com/doi/10.1002/rcm.6807/abstract;jsessionid=E87F5B7042A4C52C7B39FAE4D64C42F6.f01t02

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