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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2014

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Nuclear Aggregates of Polyamines in a radiation-induced DNA damage model

Anno di pubblicazione: 2014

Formato: Elettronico Cartaceo

Autori: Giuseppe Iacomino, Gianluca Picariello, Ilaria Stillitano, Luciano D'Agostino

Affiliazioni autori: ISA-CNR

Autori CNR:

  • LUCIANO D'AGOSTINO
  • GIUSEPPE IACOMINO
  • GIANLUCA PICARIELLO

Lingua: inglese

Abstract: Polyamines (PA) are believed to protect DNA minimizing the effect of radiation damage either by inducing DNA compaction and aggregation or acting as scavengers of free radicals. Using an in vitro pDNA double strand breakage assay based on gel electrophoretic mobility, we compared the protective capability of PA against ?-radiation with that of compounds generated by the supramolecular self-assembly of nuclear polyamines and phosphates, named Nuclear Aggregates of Polyamines (NAPs). Both unassembled PA and in vitro produced NAPs (ivNAPs) were ineffective in conferring pDNA protection at the sub-mM concentration. Single PA showed an appreciable protective effect only at high (mM) concentrations. However, concentrations of spermine (4+) within a critical range (0.48 - 1 mM) induced pDNA precipitation, an event that was not observed with NAPs-pDNA interaction. We conclude that the interaction of individual PA is ineffective to assure DNA protection, simultaneously preserving the flexibility and charge density of the double strand. Furthermore, data obtained by testing polyamine and ivNAPS with the current radiation-induced DNA damage model support the concept that PA-phosphate aggregates are the only forms through which PA interact with DNA

Lingua abstract: inglese

Pagine da: 11

Pagine a: 19

Rivista:

International journal of biochemistry & cell biology Pergamon,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 1357-2725

Numero volume: 47

DOI: 10.1016/j.biocel.2013.11.007

Referee: Sì: Internazionale

Indicizzato da: PubMed [24291171]

Parole chiave:

  • Polyamines
  • Phosphates
  • Nuclear aggregates of polyamines
  • DNA damage gamma-radiation

URL: http://www.sciencedirect.com/science/article/pii/S1357272513003452

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