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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2002

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Plasma leptin measurements in epidemiological investigation: comparison of two commonly used assays and estimate of regression dilution bias.

Anno di pubblicazione: 2002

Autori: R Iacone, O Russo, P Russo, A Venezia, V Varriale, P Strazzullo

Affiliazioni autori: Dept. of Clin. and Exp. Medicine, Federico II Univ. Medical School, Naples, Italy Dept. Biochem. Med. Biotechnologies, Federico II Univ. Medical School, Naples, Italy Institute of Food Sciences, National Research Council, Avellino, Italy

Autori CNR:

  • PAOLA RUSSO

Lingua: inglese

Abstract: Background and Aim: As leptin is the object of intensive clinical research, we compared the radio-immunological assay (RIA) and enzyme-linked immunosorbent assay (ELISA) commercially available for measuring its plasma concentration in humans (Study 1), and sought to determine the power of a single plasma leptin measurement to characterise adequately a subject within a population on the basis of its intra- and inter-individual variations (Study 2). Methods and Results: Study 1 - Plasma leptin concentrations were determined by means of RIA and ELISA in a sample of 80 males. The measurements obtained using the t\vo methods were closely correlated (r=0.942), but the bias of the means was 21.1ą73.5% (MąSD, p<0.001) and indicated that the two assays were not in agreement with each other. As expected, there were strong statistical associations between plasma leptin and a number of anthropometric indices, but the slopes of the regression of leptin concentration was significantly steeper when measured by ELISA. Study 2 - ELISA was used to measure plasma leptin concentrations in three different samples obtained from 12 males and 12 females at two-week intervals. The inter-individual variation in plasma leptin was much greater than its intra-individual variation (the ratio of intra- to inter-individual variance=0.05 and 0.04 in males and females, respectively), thus suggesting that a single fasting measurement is sufficient to characterise an individual's plasma leptin level within a population. Conclusions: ELISA is at least as effective as RIA in measuring plasma leptin, and is fully suitable for epidemiological investigations. A single measurement made in the morning and under fasting conditions is sufficient to characterise an individual within a population.

Lingua abstract: inglese

Pagine da: 71

Pagine a: 79

Rivista:

NMCD. Nutrition Metabolism and Cardiovascular Diseases Medikal Press.
Paese di pubblicazione: Italia
Lingua: inglese
ISSN: 0939-4753

Numero volume: 12

Referee: Sė: Internazionale

Indicizzato da: PubMed [12189906]

Parole chiave:

  • Enzyme-linked immunosorbent assay; Human plasma leptin measurement
  • Intra- and inter-individual variability
  • Method comparison
  • Radio-immunoassay

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