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Contributo in rivista
Tipo: Articolo in rivista
Titolo: Blood cells as a source of transcriptional biomarkers of childhood obesity and its related metabolic alterations. Results of the IDEFICS study.
Anno di pubblicazione: 2012
Autori: Sánchez J, Priego Y, Picó C, Ahrens W, De Henauw S, Mårild S, Molnár D, Moreno L, Russo P, Siani A, Tornaritis M, Veidebaum T, Palou A
Affiliazioni autori: Laboratory of Molecular Biology (J.S., T.P., C.P., A.P.), Nutrition and Biotechnology (Nutrigenomics), University of the Balearic Islands and CIBER Fisiopatología de la Obesidad y Nutricio´ n (CIBERobn), E- 07122 Palma de Mallorca, Spain; Bremen Institute for Prevention Research and Social Medicine (W.A., J.P.), University of Bremen, D-28359 Bremen, Germany; Department of Public Health/Department of Movement and Sport Sciences (S.D.H.), Faculty of Medicine and Health Sciences, Ghent University, B-9000 Ghent, Belgium; Laboratoriumsmedizin Dortmund (A.F.), Eberhard & Partner, D-44137 Dortmund, Germany; Queen Silvia Children's Hospital (S.M.), Department of Pediatrics, Go¨ teburg University, S-405 30 Gothenburg, Sweden; Department of Pediatrics (D.M.), University of Pe´ cs, H-7622 Pe´ cs, Hungary; GENUD (Growth, Exercise, Nutrition, and Development) Research Group (L.A.M.), E.U. Ciencias de la Salud, University of Zaragoza, E-50009 Zaragoza, Spain; Institute of Food Sciences (P.R., A.S.), Unit of Epidemiology and Population Genetics, National Research Council, I-83100 Avellino, Italy; Research and Education of Child Health Institute (M.T.), 2035 Strovolos, Cyprus; and National Institute for Health Development (T.V.), Tervise Arengu Instituut, 11619 Tallinn, Estonia
Abstract: Background: IDEFICS (Identification and Prevention of Dietary- and Lifestyle-Induced Health Effects in Children and Infants Project) is a European multicenter study on childhood obesity. One of its goals is to define early biomarkers of risk associated with obesity and its comorbid conditions. Objective: We considered blood cells as a new potential source of transcriptional biomarkers for these metabolic disorders and examined whether blood cell mRNA levels of some selected genes (LEPR, INSR, CPT1A, SLC27A2, UCP2, FASN, and PPAR) were altered in overweight children and whether their expression levels could be defined as markers of the insulin-resistant or dyslipidemic state associated with overweight. Design: Blood samples were obtained from 306 normal-weight and overweight children, aged 2-9 yr, from eight different European countries. Whole-blood mRNA levels were assessed by quantitative RT-PCR. Results: LEPR, INSR, and CPT1A mRNA levels were higher in overweight compared with normalweight children (the two latter only in males), whereas SLC27A2 mRNA levels were lower in overweight children. Significant associations were also found between expression levels of LEPR, INSR, CPT1A, SLC27A2, FASN, PPAR, and different parameters, including body mass index, homeostasis model assessment index, and plasma triglycerides and cholesterol levels. These associations showed that high expression levels of CPT1A, SLC27A2, INSR, FASN, or PPAR may be indicative of a lower risk for the insulin-resistant or dyslipidemic state associated with obesity, whereas low LEPR mRNA levels appear as a marker of high low-density lipoprotein cholesterol, independently of body mass index. Conclusions: These findings point toward the possibility of using the expression levels of these genes in blood cells as markers of metabolic status and can potentially provide an early warning of a future disorder.
Lingua abstract: inglese
Pagine da: E648
Pagine a: E652
The Journal of clinical endocrinology and metabolism
Numero volume: 97
Referee: Sì: Internazionale
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