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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2012

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Maize Prolamins Resistant to Peptic-tryptic Digestion Maintain Immune-recognition by IgA from Some Celiac Disease Patients

Anno di pubblicazione: 2012

Formato: Cartaceo

Autori: Maize prolamins resistant to peptic-tryptic digestion maintain immune-recognition by IgA from some celiac disease patients.

Affiliazioni autori: F. Cabrera-Chávez : S. Iametti :M. Miriani : F. Bonomi Dipartimento di Scienze Molecolari Agroalimentari, Università degli Studi di Milano, Milano, Italy A. M. C. de la Barca Coordinación de Nutrición, Centro de Investigación en Alimentación y Desarrollo, Hermosillo, Sonora, México G. Mamone Istituto di Scienze dell'Alimentazione, CNR, Avellino, Italy A. M. C. de la Barca (*) Centro de Investigación en Alimentación y Desarrollo, Carretera a la Victoria Km 0.6, P. O. Box 1735, Hermosillo 83000( Sonora, México e-mail: amc@ciad.mx

Autori CNR:

  • GIANFRANCO MAMONE

Lingua: inglese

Abstract: Maize is used as an alternative to wheat to elaborate foodstuffs for celiac patients in a gluten-free diet. However, some maize prolamins (zeins) contain amino acid sequences that resemble the wheat gluten immunodominant peptides and their integrity after gastrointestinal proteolysis is unknown. In this study, the celiac IgA-immunoreactivity to zeins from raw or nixtamalized grains, before and after peptic/tryptic digestion was evaluated and their possible immunogenicity was investigated by in silico methods. IgA from some celiac patients with HLA-DQ2 or DQ8 haplotypes recognized two alpha-zeins even after peptic/ tryptic proteolysis. However, digestion affected zeins after denaturation, reduction, and alkylation, used for identification of prolamins as alpha-zein A20 and A30 by MS/MS sequencing. An in silico analysis indicated that other zeins contain similar sequences, or sequences that may bind even better to the HLA-DQ2/DQ8 molecules compared to the already identified ones. Results concur to indicate tharelative abundance of these zeins, along with factors affecting their resistance to proteolysis, may be of paramount clinical relevance, and the use of maize in the formulation and preparation of gluten-free foods must be reevaluated in some cases of celiac disease.

Lingua abstract: inglese

Lingua altro abstract: inglese

Pagine da: 24

Pagine a: 30

Pagine totali: 7

Rivista:

Plant Foods for Human Nutrition Springer
Paese di pubblicazione:
Lingua:
ISSN: 0921-9668

Numero volume: 67

DOI: 10.1007/s11130-012-0274-4

Referee: Sì: Internazionale

Strutture CNR:

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