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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2012

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Inactivation of ccpA and aeration affect growth, metabolite production and stress tolerance in Lactobacillus plantarum WCFS1

Anno di pubblicazione: 2012

Formato: Elettronico Cartaceo

Autori: Zotta T, Ricciardi A, Guidone A, Sacco M, Muscariello L, Mazzeo MF, Cacace G, Parente E.

Affiliazioni autori: Dipartimento di Biologia, Difesa e Biotecnologie Agro-forestali, Università degli Studi della Basilicata, Potenza, Italy Dipartimento di Scienze Ambientali, Seconda Università di Napoli, Caserta, Italy Centro di Spettrometria di Massa Proteomica e Biomolecolare, Istituto di Scienze dell'Alimentazione, CNR, Avellino, Italy

Autori CNR:

  • MARIA FIORELLA MAZZEO
  • TERESA ZOTTA

Lingua: inglese

Abstract: The growth of Lactobacillus plantarum WCFS1 and of its ?ccpA ery mutant, WCFS1-2, was compared in batch fermentations in a complex medium at controlled pH (6.5) and temperature (30 °C) with or without aeration, in order to evaluate the effect of ccpA inactivation and aeration on growth, metabolism and stress resistance. Inactivation of ccpA and, to a lesser extent, aeration, significantly affected growth, expression of proteins related to pyruvate metabolism and stress, and tolerance to heat, oxidative and cold/starvation stresses. The specific growth rate of the mutant was ca. 60% of that of the wild type strain. Inactivation of ccpA and aerobic growth significantly affected yield and production of lactic and acetic acid. Stationary phase cells were more stress tolerant than exponential phase cells with little or no effect of inactivation of ccpA or aeration. On the other hand, for exponential phase cells inactivation of ccpA impaired both heat stress and cold/starvation stress, but increased oxidative stress tolerance. For both strains, aerobically grown cells were more tolerant of stresses. Evidence for entry in a viable but non-culturable status upon prolonged exposure to cold and starvation was found. Preliminary results of a differential proteomic study further confirmed the role of ccpA in the regulation of carbohydrate catabolism and class I stress response genes and allow to gain further insight on the role of this pleiotropic regulator in metabolism and stress. This is the first study in which the impact of aerobic growth on stress tolerance of L. plantarum is evaluated. Although aerobic cultivation in batch fermentations does not improve growth it does improve stress tolerance, and may have significant technological relevance for the preservation of starter and probiotic cultures.

Lingua abstract: inglese

Pagine da: 51

Pagine a: 59

Pagine totali: 8

Rivista:

International journal of food microbiology Elsevier
Paese di pubblicazione: Paesi Bassi
Lingua: inglese
ISSN: 0168-1605

Numero volume: 155

Referee: Sì: Internazionale

Parole chiave:

  • Lactobacillus plantarum
  • CcpA
  • Carbon catabolite repression
  • Stress response
  • Aerobic growth

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