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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2012

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Bovine lactoferrin-derived peptides as novel broad-spectrum inhibitors of influenza virus.

Anno di pubblicazione: 2012

Formato: Elettronico Cartaceo

Autori: Ammendolia MG, Agamennone M, Pietrantoni A, Lannutti F, Siciliano RA, De Giulio B, Amici C, Superti F.

Affiliazioni autori: Department of Technology and Health, National Institute of Health, Rome, Italy, Department of Drug Sciences, University "G. d'Annunzio", Chieti, Italy, Proteomic and Biomolecular Mass Spectrometry Center, Institute of Food Science, CNR, Avellino, Italy, Department of Biology, University of Rome Tor Vergata, Rome, Italy

Autori CNR:

  • BEATRICE DE GIULIO
  • ROSA ANNA SICILIANO

Lingua: inglese

Abstract: Bovine lactoferrin (bLf) is a multifunctional glycoprotein that plays an important role in innate immunity against infections, including influenza. Here we have dissected bLf into its C- and N-lobes and show that inhibition of influenza virus hemagglutination and cell infection is entirely attributable to the C-lobe and that all major virus subtypes, including H1N1 and H3N2, are inhibited. By far-western blotting and sequencing studies, we demonstrate that bLf C-lobe strongly binds to the HA(2) region of viral hemagglutinin, precisely the highly conserved region containing the fusion peptide. By molecular docking studies, three C-lobe fragments were identified which inhibited virus hemagglutination and infection at fentomolar concentration range. Besides contributing to explain the broad anti-influenza activity of bLf, our findings lay the foundations for exploiting bLf fragments as source of potential anti-influenza therapeutics.

Lingua abstract: inglese

Pagine da: 12

Pagine a: 19

Pagine totali: 8

Rivista:

Pathogens and Global Health Maney Publishing
Paese di pubblicazione:
Lingua: inglese
ISSN: 2047-7724

Numero volume: 106

Numero fascicolo: 1

DOI: 10.1179/2047773212Y.0000000004

Referee: Sė: Internazionale

Stato della pubblicazione: Published version

Indicizzato da: PubMed [PMID: 22595270]

Parole chiave:

  • Influenza
  • Bovine lactoferrin
  • C-lobe
  • Peptides
  • Antiviral

Strutture CNR:

Moduli:

Allegati: Bovine lactoferrin-derived peptides as novel broad-spectrum inhibitors of influenza virus. (application/pdf)

 
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