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Contributo in rivista
Tipo: Articolo in rivista
Titolo: Bovine lactoferrin-derived peptides as novel broad-spectrum inhibitors of influenza virus.
Anno di pubblicazione: 2012
Formato: Elettronico Cartaceo
Autori: Ammendolia MG, Agamennone M, Pietrantoni A, Lannutti F, Siciliano RA, De Giulio B, Amici C, Superti F.
Affiliazioni autori: Department of Technology and Health, National Institute of Health, Rome, Italy, Department of Drug Sciences, University "G. d'Annunzio", Chieti, Italy, Proteomic and Biomolecular Mass Spectrometry Center, Institute of Food Science, CNR, Avellino, Italy, Department of Biology, University of Rome Tor Vergata, Rome, Italy
Abstract: Bovine lactoferrin (bLf) is a multifunctional glycoprotein that plays an important role in innate immunity against infections, including influenza. Here we have dissected bLf into its C- and N-lobes and show that inhibition of influenza virus hemagglutination and cell infection is entirely attributable to the C-lobe and that all major virus subtypes, including H1N1 and H3N2, are inhibited. By far-western blotting and sequencing studies, we demonstrate that bLf C-lobe strongly binds to the HA(2) region of viral hemagglutinin, precisely the highly conserved region containing the fusion peptide. By molecular docking studies, three C-lobe fragments were identified which inhibited virus hemagglutination and infection at fentomolar concentration range. Besides contributing to explain the broad anti-influenza activity of bLf, our findings lay the foundations for exploiting bLf fragments as source of potential anti-influenza therapeutics.
Lingua abstract: inglese
Pagine da: 12
Pagine a: 19
Pagine totali: 8
Pathogens and Global Health
Numero volume: 106
Numero fascicolo: 1
Referee: Sė: Internazionale
Stato della pubblicazione: Published version
Indicizzato da: PubMed [PMID: 22595270]
Allegati: Bovine lactoferrin-derived peptides as novel broad-spectrum inhibitors of influenza virus.
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