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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2012

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Effect of inactivation of ccpA and aerobic growth in Lactobacillus plantarum: A proteomic perspective.

Anno di pubblicazione: 2012

Formato: Elettronico Cartaceo

Autori: Mazzeo MF, Cacace G, Peluso A, Zotta T, Muscariello L, Vastano V, Parente E, Siciliano RA.

Affiliazioni autori: Centro di Spettrometria di Massa Proteomica e Biomolecolare, Istituto di Scienze dell'Alimentazione, CNR, Avellino, Italy Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Università della Basilicata, Potenza, Italy Dipartimento di Scienze Ambientali, Seconda Università degli Studi di Napoli, Caserta, Italy

Autori CNR:

  • MARIA FIORELLA MAZZEO
  • ROSA ANNA SICILIANO
  • TERESA ZOTTA

Lingua: inglese

Abstract: Lactobacillus plantarum is a facultative heterofermentative lactic acid bacterium widely used in the production of most fermented food due to its ability to thrive in several environmental niches, including the human gut. In order to cope with different growth conditions, it has developed complex molecular response mechanisms, characterized by the induction of a large set of proteins mainly regulated by HrcA and CtsR repressors as well as by global regulators such as carbon catabolite control protein A (CcpA). In this study, the role of CcpA in the regulation of growth under anaerobiosis and aerobiosis, and the adaptation to aeration in L. plantarum WCFS1 were comprehensively investigated by differential proteomics. The inactivation of ccpA, in both growth conditions, significantly changed the expression level of 76 proteins, mainly associated with carbohydrate and energy metabolism, membrane transport, nucleotide metabolism, protein biosynthesis and folding. The role of CcpA as pleiotropic regulator was particularly evident at the shift from homolactic fermentation to mixed fermentation. Proteomic results also indicated that the mutant strain was more responsive to aerobic growth condition.

Lingua abstract: inglese

Pagine da: 4050

Pagine a: 4061

Pagine totali: 12

Rivista:

Journal of proteomics Elsevier
Paese di pubblicazione: Paesi Bassi
Lingua: inglese
ISSN: 1876-7737

Numero volume: 75

Numero fascicolo: 13

DOI: 10.1016/j.jprot.2012.05.019.

Referee: Sì: Internazionale

Indicizzato da: PubMed [PMID: 22634038]

Parole chiave:

  • L. plantarum; Proteomics; CcpA; Aerobiosis

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