Home |  English version |  Mappa |  Commenti |  Sondaggio |  Staff |  Contattaci Cerca nel sito  
Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2000

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Tissue transglutaminase is the target in both rodent and primate tissues for celiac disease-specific autoantibodies

Anno di pubblicazione: 2000

Formato: Cartaceo

Autori: Korponay-Szab, I.R.ad, Sulkanen, S.a, Halttunen, T.a, Maurano, F.e, Rossi, M.e, Mazzarella, G.e, Laurila, K.a, Troncone, R.f, Mki, M.abcg

Affiliazioni autori: a Institute of Medical Technology, University of Tampere, Tampere, Finland b Department of Pediatrics, University of Tampere, Tampere, Finland c Medical School, University of Tampere, Tampere, Finland d Heim Pl Children's Hospital, Budapest, Hungary e Institute of Food Science and Technology, Consiglio Nazionale delle Ricerche, Avellino, Italy f Department of Pediatrics, European Laboratory for the Investigation of Food-Induced Diseases, University Federico II, Naples, Italy g Coeliac Disease Study Group, Institute of Medical Technology, University of Tampere, P.O. Box 607, FIN-33101 Tampere, Finland

Autori CNR:

  • FRANCESCO MAURANO
  • GIUSEPPE MAZZARELLA
  • MAURO ROSSI

Lingua: inglese

Abstract: Background: Endomysial antibodies have recently been shown to react with tissue transglutaminase. This study was undertaken to investigate whether the tissue distribution of transglutaminase is also compatible with reticulin, jejunal, and fibroblast autoantibody binding patterns. Methods: Sera from patients with and without celiac disease, monoclonal tissue transglutaminase antibodies, and sera from mice parenterally immunized against commercially available tissue transglutaminase, transglutaminase complexed with gliadin, or gliadin were used in indirect immunofluorescence and double-staining studies using both rodent and primate tissues as substrates. Also, antibody competition, affinity chromatography, and potassium thiocyanate extraction studies were undertaken. Results: Tissue transglutaminase antibody binding patterns were identical with the extracellular binding patterns seen with celiac patient sera. Human umbilical cord-derived fibroblasts exhibited both cytoplasmic and extracellular matrix staining. Double staining with patients' sera and tissue transglutaminase antibodies showed complete overlapping. Tissue transglutaminase effectively absorbed reticulin-endomysial antibodies from celiac sera, and patients' sera blocked the staining of the monoclonal tissue transglutaminase antibodies. Potassium thiocyanate extraction abolished the staining patterns, but they were elicited again after readdition of tissue transglutaminase. Conclusions: Reticulin, endomysial, and jejunal antibodies detect transglutaminase in both rodent and primate tissues, indicating that these tissue autoantibodies are identical.

Lingua abstract: inglese

Pagine da: 520

Pagine a: 527

Rivista:

Journal of pediatric gastroenterology and nutrition Raven Press,
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0277-2116

Numero volume: 31

Numero fascicolo: 5

DOI: 10.1097/00005176-200011000-0001

Referee: S: Internazionale

Indicizzato da: PubMed [11144437]

Parole chiave:

  • Autoantigen; Celiac disease; Endomysial antibodies; Jejunal antibodies; Reticulin antibodies; Tissue transglutaminase

Strutture CNR:

Moduli:

 
Torna indietro Richiedi modifiche Invia per email Stampa
Home Il CNR  |  I servizi News |   Eventi | Istituti |  Focus