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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2011

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Identification of glutathione-methacrylates adducts in gingival fibroblasts and erythrocytes by HPLC-MS and capillary electrophoresis

Anno di pubblicazione: 2011

Formato: Elettronico

Autori: G. Nocca, R. Ragno, V. Carbone, G. E. Martorana, D. V. Rossetti, G. Gambarini, B. Giardina, A. Lupi

Affiliazioni autori: Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy Dipartimento di Chimica e Tecnologie del Farmaco dell'Università La Sapienza, Rome, Italy Istituto di Scienze dell' Alimentazione, CNR, Avellino, Italy Department of Dental Science, Sapienza University of Rome, Rome, Italy Istituto di Chimica del Riconoscimento Molecolare, CNR, Rome, Italy

Autori CNR:

  • VIRGINIA CARBONE
  • ALESSANDRO LUPI

Lingua: inglese

Abstract: Objectives. Methacrylic monomers are released, from dental composite resins, either into the oral cavity or in pulpal tissues, where they can cause local or systemic adverse effects. The mechanisms of these effects are not well understood, probably because such molecules can act at different levels also inducing a depletion of intracellular glutathione (GSH). GSH can detoxify methacrylates by conjugating their ,-unsaturated carbon-carbon moiety to the thiol group, with the catalysis of glutathione S-transferases (GST). This reaction determines a GSH cellular depletion and belongs to the metabolism of ,-unsaturated esters, protecting the body against the toxic effects of electrophiles. On the basis of the above considerations, this work aim is to set up a method for the detection of the adducts formed by methacrylic monomers with GSH in cells using HPLC coupled to mass spectrometry (HPLC-MS) and micellar electrokinetic capillary chromatography (MECK) techniques. Methods and results. Adducts of glutathione with triethylene glycol dimethacrylate (TEGDMA) and hydroxyethyl methacrylate (HEMA) were incontrovertibly identified by HPLC-MS and MECK in human gingival fibroblasts and erythrocytes, both outside and inside cells. Molecular docking simulations ofHEMAand TEGDMA in the experimental structure of glutathione S-transferase, are also reported to rationalize the effectiveness of such enzyme in the catalysis of the above described reaction. Significance. The setup of a method for the identification of GSH-methacrylate adducts allows to determine when the metabolic pathway involving such compounds is employed by cells for the detoxification of monomers leached from composite resins.

Lingua abstract: inglese

Pagine da: e87

Pagine a: e98

Rivista:

Dental materials Munksgaard,
Paese di pubblicazione: Paesi Bassi
Lingua: inglese
ISSN: 0109-5641

Numero volume: 27

Referee: Sì: Internazionale

Indicizzato da: PubMed [PMID: 21371745]

Parole chiave:

  • Methacrylic monomers
  • GSH
  • Metabolism
  • HPLC-MS
  • Capillary electrophoresis

Strutture CNR:

Moduli:

Allegati: Identification of glutathione-methacrylates adducts in gingival fibroblasts and erythrocytes by HPLC-MS and capillary electrophoresis (application/pdf)

 
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