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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2008

Contributo in rivista

Tipo: Abstract in rivista

Titolo: Effects of Lactobacillus casei administration in a mouse model of gluten hypersensitivity

Anno di pubblicazione: 2008

Formato: Cartaceo

Autori: D'Arienzo R, Maurano F, Luongo D, Stefanile R, Mazzarella G, Bergamo P, Ricca E, Rossi M.

Affiliazioni autori: Istituto di Scienze dell'Alimentazione Università degli Studi di Napoli

Autori CNR:

  • PAOLO BERGAMO
  • ROSSANA D'ARIENZO
  • DIOMIRA LUONGO
  • FRANCESCO MAURANO
  • GIUSEPPE MAZZARELLA
  • MAURO ROSSI

Lingua: inglese

Abstract: Introduction: Celiac disease (CD), is the most common food-sensitive enteropathy in humans, caused by the lack of oral tolerance to wheat gluten (1). To study this disease we use a transgenic mouse model expressing the HLA-DQ8 molecule in the absence of endogenous class II genes (2). The adjuvant function Lactobacillus casei is well known (3). In this study we analyzed the effects of oral administration of L. casei in DQ8 transgenic mice following mucosal sensitization with gliadin along with cholera toxin. Methods: DQ8 transgenic mice were administered intragastrically with 500 mg of a chymotryptic digest of gliadin (ct-gliadin) along with 25 ?g of cholera toxin (CT) on days 0, 7 and 14. In someexperiments L. casei (1x1010/dose, four doses/week) was co-administered per os. On day 21 mice were sacrificed. Spleen and MLN were isolated for in vitro assessment of gliadin-specific immunity. Small intestine fragments were collected for RNA analysis and immunomorphometric measurements. Results: Co-administration of L.casei in gliadin-sensitive DQ8 transgenic mice caused a strong increase of the intestinal gliadin-specific cell mediated immune response (SI: 2.75 ± 0.1 vs 4.4 ± 0.03; control vs L. casei; p <0.05) as well as the IFNg? response (pg/ml: 3976 ± 212 vs 9891 ± 2649; control vs L. casei; p < 0.05). Probiotic administrationwas also associated to a Th1 polarization of the small intestinal mucosa. However, the strong increase in the IFN-g production did not provoke a gliadin-specific enteropathy. Conclusion: Exacerbation of the gliadin-specific Th1-like immune response by L. casei is not associated to induction of enteropathy in our model of CD. Other parameters are then required to induce the mucosal damage. From this point of view administration of L. casei can be considered safe also in an ongoing intestinal hypersensitivity to food antigens References: (1) Maki, M., K. Mustalahti, J. Kokkonen, P. Kulmala, M. Haapalahti, T. Karttunen, J. Ilonen, K. Laurila, I. Dahlbom, T. Hansson, P. Hopfl, and M. Knip. 2003. Prevalence of Celiac disease among children in Finland. N. Engl. J. Med. 348: 2517-2524; (2) Cheng, S., J. Baisch, C. Krco, S. Savarirayan, J. Hanson, K. Hodgson, M. Smart, and C. David. 1996. Expression and function of HLA-DQ8 DQA1*0301/DQB1*0302) genes in 23 transgenic mice. Eur. J. Immunogenet. 23: 15-20; (3) Maldonado Galdeano and Perdigon, 2006 "The Probiotic Bacterium Lactobacillus casei Induces Activation of the Gut Mucosal Immune System through Innate Immunity" Clin.Vaccine Immunol 13:219-226

Pagine da: 171

Pagine a: 190

Rivista:

International journal of probiotics & prebiotics New Century Health Publishers,
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 1555-1431

Numero volume: 3

Referee: Sì: Internazionale

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