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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2012

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Short wheat challenge is a reproducible in-vivo assay to detect immune response to gluten.

Anno di pubblicazione: 2012

Autori: Camarca A, Radano G, Di Mase R, Terrone G, Maurano F, Auricchio S, Troncone R, Greco L, Gianfrani C.

Affiliazioni autori: a Institute of Food Sciences-CNR, Avellino, Italy b Department of Paediatrics, European Laboratory for the Investigation of Food-Induced Diseases, University of Naples, Naples, Italy

Autori CNR:


Lingua: inglese

Abstract: It has been reported that interferon (IFN)-?-secreting T cells reactive to gluten can be detected in the peripheral blood of individuals with treated coeliac disease (CD) after a short consumption of wheat-containing food. By contrast, very little is known about the reproducibility of this in-vivo procedure in the same patient cohort which underwent two, or more, gluten consumptions. Fourteen coeliac patients in remission consumed wheat bread for 3 days; 13 underwent a second gluten challenge after a wash-out of 3-10 months on a strict gluten-free diet. Immune reactivity to gluten was analysed in peripheral blood by detecting IFN-? before and 6 days after commencing a gluten diet. Gliadin-specific IFN-?-secreting CD4 + T cells increased significantly on day 6 of the first challenge. These cells resulted as prevalently human leucocyte antigen (HLA)-DQ restricted and with a phenotype of gut homing, as suggested by the expression of ?7-integrin. Similarly, reactiveness to gliadin was observed after the second wheat consumption, although with an individual variability of responses at each challenge. Our findings confirmed that the short wheat challenge is a non-invasive approach to investigate the gluten-related immune response in peripheral blood of subjects intolerant to gluten. Furthermore, we demonstrated that the in-vivo procedure can be reproduced in the same subject cohort after a gluten wash-out of at least 3 months. Our study has important implications for the application of this procedure to clinical practice

Lingua abstract: inglese

Pagine da: 129

Pagine a: 136


Clinical and experimental immunology Blackwell Scientific,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0009-9104

Numero volume: 169

Numero fascicolo: 2

DOI: 10.1111/j.1365-2249.2012.04597.x

Referee: Sì: Internazionale

Indicizzato da: PubMed [22774987]

Parole chiave:

  • In-vivo challenge; Interferon-?; Peripheral blood; Wheat gluten

Strutture CNR:


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