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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2013

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Selective inhibition of the gliadin-specific, cell-mediated immune response by transamidation with microbial transglutaminase.

Anno di pubblicazione: 2013

Formato: Cartaceo

Autori: Lombardi E, Bergamo P, Maurano F, Bozzella G, Luongo D, Mazzarella G, Rotondi Aufiero V, Iaquinto G, Rossi M.

Affiliazioni autori: Institute of Food Sciences, National Research Council, Avellino, Italy;

Autori CNR:

  • PAOLO BERGAMO
  • DIOMIRA LUONGO
  • FRANCESCO MAURANO
  • GIUSEPPE MAZZARELLA
  • MAURO ROSSI
  • VERA ROTONDI AUFIERO

Lingua: inglese

Abstract: CD is an immune-mediated enteropathy caused by the ingestion of wheat gluten. The modification of gluten by intestinal tTGase plays a crucial role in CD pathogenesis. In this study, we observed that extensive transamidation of wheat flour with K-C(2)H(5) by mTGase yielded spf and K-gliadins fractions. By Western blot, we found that these modifications were associated with strongly reduced immune cross-reactivity. With the use of DQ8 tg mice as a model of gluten sensitivity, we observed a dramatic reduction in IFN? production in gliadin-specific spleen cells challenged with spf and K-gliadins in vitro (n=12; median values: 813 vs. 29 and 99; control vs. spf and K-gliadins, P=0.012 for spf, and P=0.003 for K-gliadins). For spf, we also observed an increase in the IL-10/IFN? protein ratio (n=12; median values: 0.3 vs. 4.7; control vs. spf, P=0.005). In intestinal biopsies from CD patients challenged in vitro with gliadins (n=10), we demonstrated further that K-gliadins dramatically reduced the levels of antigen-specific IFN? mRNA in all specimens responsive to native gliadins (four of 10; P<0.05). As cytotoxic effects have been described for gliadins, we also studied GST and caspase-3 activities using the enterocytic Caco-2 cell line. We found that neither activities were modified by flour transamidation. Our results indicate that K-C(2)H(5) cross-linking via mTGase specifically affects gliadin immunogenicity, reversing the inducible inflammatory response in models of gluten sensitivity without affecting other aspects of the biological activity of gliadins.

Lingua abstract: inglese

Pagine da: 479

Pagine a: 488

Rivista:

Journal of leukocyte biology Liss,
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0741-5400

Numero volume: 93

Numero fascicolo: 4

Referee: Sì: Internazionale

Indicizzato da: PubMed [23108099]

Strutture CNR:

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