Home |  English version |  Mappa |  Commenti |  Sondaggio |  Staff |  Contattaci Cerca nel sito  
Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2012

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Diversity, Molecular Characterization and Expression of T Cell Receptor in a Teleost Fish, the Sea Bass (Dicentrarchus labrax, L).

Anno di pubblicazione: 2012

Formato: Elettronico

Autori: Buonocore F, Castro R, Randelli E, Lefranc MP, Six A, Kuhl H, Reinhardt R, Facchiano A, Boudinot P, Scapigliati G.

Affiliazioni autori: Adrien Six Centre National Recherche Scientifique, Unité Mixte de Recherches, "Immunology, Immunopathology, Immunotherapy", Paris, France Rosario Castro, Pierre Boudinot Institut National de la Recherche Agronomique, Unité de Virologie et Immunologie Moléculaires, Jouy-en-Josas, Paris, France Heiner Kuhl Max Planck Institute for Molecular Genetics, Berlin, Germany Marie-Paule Lefranc The International ImMunoGeneTics Information System®, Laboratoire d'ImmunoGénétique Moléculaire, Institut de Génétique Humaine, Centre National de la Recherche Scientifique and Université Montpellier 2, Montpellier, France Richard Reinhardt Genome Centre at Max Planck Institute for Plant Breeding Research, Cologne, Germany Adrien Six: Université Pierre et Marie Curie (Université Paris-06), Unité Mixte de Recherches 7211, "Integrative Immunology" Team, Paris, France Francesco Buonocore, Elisa Randelli, Giuseppe Scapigliati: Department for Innovation in Biological, Agro-Food and Forest Systems, University of Tuscia, Largo dell'Università, Viterbo, Italy Angelo Facchiano: Laboratory of Bioinformatics and Computational Biology - National Research Council, Istitute of Sciences of Alimentation, Avellino, Italy

Autori CNR:


Lingua: inglese

Abstract: Two lineages of T cells, expressing either the ?? T cell receptor (TR) or the ?? TR, exist in Gnathostomes. The latter type of T cells account for 1-10 % of T cells in blood and up to 30 % in the small intestine. They may recognize unconventional antigens (phosphorylated microbial metabolites, lipid antigens) without the need of major histocompatibility class I (MH1) or class II (MH2) presentation. In this work we have described cloning and structural characterization of TR -chain (TRG) from the teleost Dicentrarchus labrax. Further, by means of quantitative PCR analysis, we analyzed TRG expression levels both in poly I:C stimulated leukocytes in vitro, and following infection with betanodavirus in vivo. Two full length cDNAs relative to TRG, with the highest peptide and nucleotide identity with Japanese flounder, were identified. A multiple alignment analysis showed the conservation of peptides fundamental for TRG biological functions, and of the FGXG motif in the FR4 region, typical of most TR and immunoglobulin light chains. A 3D structure consisting of two domains mainly folded as beta strands with a sandwich architecture for each domain was also reported. TRG CDR3 of 8-18 AA in length and diversity in the TRG rearrangements expressed in thymus and intestine for a given V/C combination were evidenced by junction length spectratyping. TRG mRNA expression levels were high in basal conditions both in thymus and intestine, while in kidney and gut leukocytes they were up-regulated after in vitro stimulation by poly I:C. Finally, in juveniles the TRG expression levels were up-regulated in the head kidney and down-regulated in intestine after in vivo infection with betanodavirus. Overall, in this study the involvement of TRG-bearing T cells during viral stimulation was described for the first time, leading to new insights for the identification of T cell subsets in fish.

Lingua abstract: inglese


PloS one Public Library of Science
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 1932-6203

DOI: 10.1371/journal.pone.0047957

Referee: Sì: Internazionale

Stato della pubblicazione: Published version

Indicizzato da: PubMed [23133531]

Parole chiave:

  • T cell receptor; Teleost Fish; viral stimulation

Strutture CNR:


Torna indietro Richiedi modifiche Invia per email Stampa
Home Il CNR  |  I servizi News |   Eventi | Istituti |  Focus