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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2012

Contributo in rivista

Tipo: Articolo in rivista

Titolo: DNA and nuclear aggregates of polyamines

Anno di pubblicazione: 2012

Formato: Elettronico Cartaceo

Autori: Giuseppe Iacomino , Gianluca Picariello , Luciano D'Agostino

Affiliazioni autori: Istituto di Scienze dell'Alimentazione, Consiglio Nazionale delle Ricerche, via Roma 64, 83100 Avellino, Italy

Autori CNR:

  • GIUSEPPE IACOMINO
  • GIANLUCA PICARIELLO

Lingua: inglese

Abstract: Polyamines (PAs) are linear polycations that are involved in many biological functions. Putrescine, spermidine and spermine are highly represented in the nucleus of eukaryotic cells and have been the subject of decades of extensive research. Nevertheless, their capability to modulate the structure and functions of DNA has not been fully elucidated. We found that polyamines self-assemble with phosphate ions in the cell nucleus and generate three forms of compounds referred to as Nuclear Aggregates of Polyamines (NAPs), which interact with genomic DNA. In an in vitro setting that mimics the nuclear environment, the as- sembly of PAs occurs within well-defined ratios, independent of the presence of the DNA template. Strict structural and functional analogies exist between the in vitro NAPs (ivNAPs) and their cellular homologues. Atomic force microscopy showed that ivNAPs, as theoretically predicted, have a cyclic structure, and in the presence of DNA, they form a tube-like arrangement around the double helix. Features of the interaction be- tween ivNAPs and genomic DNA provide evidence for the decisive role of "natural" NAPs in regulating impor- tant aspects of DNA physiology, such as conformation, protection and packaging, thus suggesting a new vision of the functions that PAs accomplish in the cell nucleus.

Lingua abstract: inglese

Pagine da: 1745

Pagine a: 1755

Rivista:

Biochimica et biophysica acta. Molecular cell research Elsevier
Paese di pubblicazione: Paesi Bassi
Lingua: inglese
ISSN: 0167-4889

Numero volume: 1823

DOI: 10.1016/j.bbamcr.2012.05.033

Referee: Sė: Internazionale

Parole chiave:

  • nuclear aggregates of polyamines
  • genomic DNA
  • DNA packaging
  • self-assembly
  • supramolecular chemistry

Strutture CNR:

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