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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2009

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Matrix/analyte ratio influencing polymer molecular weight distribution in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Anno di pubblicazione: 2009

Formato: Elettronico Cartaceo

Autori: Schlosser, G.; Jakab, A.; Pocsfalvi, G.; Vekey, K.; Hudecz, F.; Mezo, G.,

Affiliazioni autori: Chemical Research Center - Hungarian Academy of Sciences; Research Group of Peptide Chemistry - Hungarian Academy of Sciences; Proteomic and Biomolecular Mass Spectrometry Centre - Institute of Food Science and Technology; Department of Organic Chemistry - Eötvos L. University

Autori CNR:


Abstract: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has been used to characterize poly(L-lysine) polymers and unique oligomer peptides, like 10-, 15- and 25-mer [LyS](n) oligolysine peptides. Several matrices have been tried in order to find optimal conditions, but only alpha-cyano-4-hydroxycinnamic acid gave analytically useful spectra. The synthetic oligomers and their mixtures gave good quality spectra, showing protonated and cationized molecules, including doubly charged species. The polymers, analogously, gave a wide distribution of single- and double-cationized peak series. The polymer distributions observed indicate the presence of significant suppression effects. The concentration (matrix/analyte ratio) was found to influence the results significantly; distributions shifting to higher masses when higher polymer concentrations were used. This effect was studied in detail using the synthetic ('monodisperse') oligolysine peptides. It was found that the relative intensities change by over an order of magnitude in the 0.1-10 pmol/mu L concentration range (typical for most proteomic analyses). The results indicate that concentration effects need to be considered when MALDI-MS is used for quantitative purposes.

Pagine da: 1249

Pagine a: 1254


RCM. Rapid communications in mass spectrometry Heyden,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0951-4198

Numero volume: 23

Numero fascicolo: 9

DOI: 10.1002/rcm.3993

Referee: Sě: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000265542300004]

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