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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2008

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Mass spectrometric and linear discriminant analysis of N-glycans of human serum alpha-1-acid glycoprotein in cancer patients and healthy individuals

Anno di pubblicazione: 2008

Formato: Elettronico Cartaceo

Autori: Imre Timea; Kremmer Tibor; Heberger Karoly; Molnar-Szollosi Eva; Ludanyi Krisztina.; Pocsfalvi Gabriella; Malorni Antonio; Drahos Laszlo; Vekey Karoly

Affiliazioni autori: Institute of Structural Chemistry, Chemical Research Center, Hungarian Academy of Sciences, Budapest, Pusztaszeri u.59-67, Hungary Institute of Biomolecular Chemistry, Chemical Research Center of the Hungarian Academy of Sciences, Budapest, Pusztaszeri u.59-67, Hungary Department of Biochemistry, National Institute of Oncology, H-1122 Budapest, Hungary Istituto di Scienze dell' Alimentazione, Consiglio Nazionale delle Ricerche, Avellino, Via Roma 52 a-c. 83100, Italy

Autori CNR:

  • ANTONIO MALORNI
  • GABRIELLA KATALIN POCSFALVI

Lingua: inglese

Abstract: N-glycan oligosaccharides of human serum ?1-acid glycoprotein (AGP) samples isolated from 43 individuals (healthy individuals and patients with lymphoma and with ovarian tumor) were analyzed by MALDI-TOF mass spectrometry and a multivariate statistical method (linear discriminant analysis, LDA). 34 different glycan structures have been identified. From the glycosylation pattern determined by mass spectrometry fucosylation and branching indices have been calculated. These parameters show only small differences between the patient groups studied, but these differences are not sufficiently large to use as a potential biomarker. LDA analysis, on the other hand shows a very good separation between the three groups (with a classification of 88%). Cross-validation indicates that the method has predictive power: Identifying cancerous vs. healthy individuals shows 96% selectivity and 93% specificity; identification of lymphoma vs. the mixed group of healthy and ovarian tumor cases is also promising (72% selectivity and 84% specificity). The pilot study presented here demonstrates that mass spectrometry combined with linear discriminant analysis (LDA) may provide valuable data for identifying and studying the pathophysiology of malignant diseases.

Pagine da: 186

Pagine a: 197

Rivista:

Journal of proteomics Elsevier
Paese di pubblicazione: Paesi Bassi
Lingua: inglese
ISSN: 1876-7737

Numero volume: 71

Numero fascicolo: 2

DOI: 10.1016/j.jprot.2008.04.OOS

Referee: Sė: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000260816400006]

Parole chiave:

  • biomarker research
  • N-glycans
  • AGP

URL: http://www.ncbi.nlm.nih.gov/pubmed/18617146

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