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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2009

Contributo in rivista

Tipo: Articolo in rivista

Titolo: The obesity and inflammatory marker haptoglobin attracts monocytes via interaction with chemokine (C-C motif) receptor 2 (CCR2).

Anno di pubblicazione: 2009

Formato: Elettronico

Autori: Maffei M; Funicello M; Vottari T; Gamucci O; Costa M; Lisi S; Viegi A; Ciampi O; Bardi G; Vitti P; Pinchera A; Santini F

Affiliazioni autori: Dulbecco Telethon Institute (Maffei, Funicello, Vottari, Lisi) Istituto di Neuroscienze, CNR (Costa, Bardi) Scuola Normale Superiore, Pisa, Italy (Viegi) Dip Psichiatria, Neurobiologia, Farmacologia, Univ Pisa (Ciampi) Dip Endocrinologia e Ren, University Hospital of Pisa (Maffei, Gamucci, Vitti Santini Pinchera)

Autori CNR:


Lingua: inglese

Abstract: Background Obesity is a chronic low inflammatory state. In the obesity condition the white adipose tissue (WAT) is massively infiltrated with monocytes/macrophages, and the nature of the signals recruiting these inflammatory cells has yet to be fully elucidated. Haptoglobin (Hp) is an inflammatory marker and its expression is induced in the WAT of obese subjects. In an effort to elucidate the biological significance of Hp presence in the WAT and of its upregulation in obesity we formulated the hypothesis that Hp may serve as a macrophage chemoattractant. Results We demonstrated by chemotaxis assay that Hp is able to attract chemokine (C-C motif) receptor 2 (CCR2)-transfected pre-B lymphocytes and monocytes in a dose-dependent manner. Moreover, Hp-mediated migration of monocytes is impaired by CCR2-specific inhibition or previous cell exposure to monocyte chemoattractant protein 1 (MCP1) (also known as CCR2 ligand or chemokine (C-C motif) ligand 2 (CCL2)). Downstream effects of Hp/CCR2 interaction were also investigated: flow cytometry proved that monocytes treated with Hp show reduced CCR2 expression on their surface; Hp interaction induces calcium release that is reduced upon pretreatment with CCR2 antagonist; extracellular signal-regulated kinase (ERK)1/2, a signal transducer activated by CCR2, is phosphorylated following Hp treatment and this phosphorylation is reduced when cells are pretreated with a specific CCR2 inhibitor. Consistently, blocking the ERK1/2 pathway with U0126, the selective inhibitor of the ERK upstream mitogen-activated protein (MAP)-ERK kinase (MEK), results in a dramatic reduction (by almost 100%) of the capability of Hp to induce monocyte migration. Conclusions Our data show that Hp is a novel monocyte chemoattractant and that its chemotactic potential is mediated, at least in part. by its interaction with CCR2.

Lingua abstract: inglese

Pagine da: 87

Pagine a: 101

Pagine totali: 14


BMC biology BioMed Central,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 1741-7007

Numero volume: 7

DOI: 10.1186/1741-7007-7-87

Referee: Ś: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000273818600001]

Parole chiave:

  • Chemotaxis
  • Inflammation
  • Chemokine
  • monocytes

URL: http://www.biomedcentral.com/1741-7007/7/87

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Allegati: Articolo pubblicato (application/pdf)

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