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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2011

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Expression of the Lactobacillus plantarum malE gene is regulated by CcpA and a MalR-like protein.

Anno di pubblicazione: 2011

Formato: Elettronico Cartaceo

Autori: Muscariello L; Vastano V; Siciliano RA; Sacco M; Marasco R.

Affiliazioni autori: Dipartimento di Scienze Ambientali, Seconda Università di Napoli, via Vivaldi 43, 81100 Caserta, Italy Centro di Spettrometria di Massa Proteomica e Biomolecolare, Istituto di Scienze dell'Alimentazione, CNR, Avellino, Italy Dipartimento di Scienze della Vita, Seconda Università di Napoli, via Vivaldi 43, 81100 Caserta, Italy

Autori CNR:

  • ROSA ANNA SICILIANO

Lingua: inglese

Abstract: Lactobacillus plantarum is commonly used in the food industry as a starter in various fermentations, especially in vegetable fermentations, in which starch is a common substrate. This polysaccharide, which is obtained from potatoes or corn and is hydrolysed mainly to maltose and glucose by acids or enzymes, is commercially used for the production of lactate by lactic acid fermentation. In this study, we describe the regulation of malE gene expression in L. plantarum. This gene, located in a 7-gene cluster, probably organized in an operon, encodes a putative maltose/maltodextrin-binding protein. We studied the expression of malE in L. plantarum LM3 (wild type) and in LM3-2 (ccpA1), which carries a null mutation in the ccpA gene, encoding the catabolite control protein A (CcpA). In the presence of glucose, expression of the MalE protein was higher in the mutant strain as compared to that in the wild-type strain. Transcription of the malE gene was induced by maltose and regulated by a CcpA-mediated carbon catabolite repression. Further, we isolated strains carrying mutations in 2 genes, lp_0172 and lp_0173, whose deduced amino acid sequences share significant identity with MalR, a regulator of the maltose operon in several gram-positive bacteria. A double mutant exhibited glucose-insensitive malE transcription, while absence of the functional Lp_0172 open reading frame had no effect on malE expression.

Lingua abstract: inglese

Pagine da: 950

Pagine a: 955

Pagine totali: 6

Rivista:

Journal of microbiology Microbiological society of Korea
Paese di pubblicazione: Corea del Sud
Lingua: inglese
ISSN: 1225-8873

Numero volume: 49

Numero fascicolo: 6

DOI: 10.1007/s12275-011-0495-5

Referee: Sì: Internazionale

Indicizzato da:

  • ISI Web of Science (WOS) [000298602600011]
  • PubMed [22203558]

Parole chiave:

  • maltose/maltodextrin
  • Lactobacillus plantarum
  • catabolite control protein A
  • MalR

Strutture CNR:

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