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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2011

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Proteomic Profiling of Human Melanoma Metastatic Cell Line Secretomes

Anno di pubblicazione: 2011

Formato: Cartaceo

Autori: Micaela Rocco; Livia Malorni;Rosaria Cozzolino; Giuseppe Palmieri;Carla Rozzo; Antonella Manca; Augusto Parente; Angela Chambery

Affiliazioni autori: Department of Life Sciences, Via Vivaldi 43, Second University of Naples, I-81100 Caserta, Italy Proteomic and Biomolecular Mass Spectrometry Center, Institute of Food Science and Technology, National Research Council (CNR), Via Roma 64, I-83100 Avellino, Italy Unit of Cancer Genetics, Institute of Biomolecular Chemistry, National Research Council (CNR), Traversa La Crucca 3, Baldinca Li Punti, I-07100 Sassari, Italy

Autori CNR:

  • ROSARIA COZZOLINO
  • ANTONELLA MANCA
  • GIUSEPPE PALMIERI
  • CARLA MARIA ROZZO

Lingua: inglese

Abstract: During the last few years, the incidence and mortality of human melanoma have rapidly increased. Metastatic spread of malignant melanoma is often associated with cancer progression with poor prognosis and survival. These processes are controlled by dynamic interactions between tumor melanocytes and neighboring stromal cells, whose deregulation leads to the acquisition of cell proliferation capabilities and invasiveness. It is increasingly clear that a key role in carcinogenesis is played by secreted molecules either by tumor and surrounding stromal cells. To address the issue of the proteins secreted during cancer progression, the proteomic profiling of secretomes of cancer cell lines from different melanoma metastases of the same patient (PE-MEL-41, PE-MEL-47, and PE-MEL-43) was performed by applying a shotgun LCMS/MS-based approach. The results provide a list of candidate proteins associated with the metastatic potential of PE-MEL melanoma cell lines. Among them, several matricellular proteins previously reported as involved in melanoma aggressiveness were identified (i.e., SPARC, osteopontin). In addition, the extracellular matrix protein 1 that stimulates proliferation and angiogenesis of endothelial cells as well as the fibronectin, involved in cell adhesion and motility, were identified. The present work provides the basis to clarify the complex extracellular protein networks implicated in human melanoma cell invasion, migration, and motility.

Lingua abstract: inglese

Pagine da: 4703

Pagine a: 4714

Rivista:

Journal of proteome research American Chemical Society,
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 1535-3893

Numero volume: 10

DOI: 10.1021/pr200511f

Referee: Sė: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000295602700029]

Parole chiave:

  • melanoma
  • metastasis
  • LC

Strutture CNR:

Moduli:

Allegati: Proteomic Profiling of Human Melanoma Metastatic Cell Line Secretomes (application/pdf)

 
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