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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2011

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Conjugated linoleic acid protects against gliadin-induced depletion of intestinal defenses.

Anno di pubblicazione: 2011

Formato: Elettronico Cartaceo

Autori: Bergamo P; Gogliettino M; Palmieri G; Cocca E; Maurano F; Stefanile R; Balestrieri M; Mazzarella G; David C; Rossi M.

Affiliazioni autori: CNR-ISA ; CRR-IBBR; Mayo Clinic College of Medicine, Rochester, MN, USA

Autori CNR:


Lingua: inglese

Abstract: SCOPE: The involvement of oxidative stress in gluten-induced toxicity has been evidenced in vitro and in clinical studies but has never been examined in vivo. We recently demonstrated the protective activity of conjugated linoleic acid (CLA), which functions by the activation of nuclear factor erythroid 2-related factor2 (Nrf2), a key transcription factor for the synthesis of antioxidant and detoxifying enzymes (phase 2). Here, we evaluate the involvement of nuclear factor erythroid 2-related factor2 in gliadin-mediated toxicity in human Caco-2 intestinal cells and in gliadin-sensitive human leukocyte antigen-DQ8 transgenic mice (DQ8) and the protective activity of CLA. METHODS AND RESULTS: Gliadin effects in differentiated Caco-2 cells and in DQ8 mice, fed with a gliadin-containing diet with or without CLA supplementation, were evaluated by combining enzymatic, immunochemical, immunohistochemical, and quantitative real-time PCR (qRT-PCR) assays. Gliadin toxicity was accompanied by downregulation of phase 2 and elevates proteasome-acylpeptide hydrolase activities in vitro and in vivo. Notably, gliadin was unable to generate severe oxidative stress extent or pathological consequences in DQ8 mice intestine comparable to those found in celiac patients and the alterations produced were hampered by CLA. CONCLUSION: The beneficial effects of CLA against the depletion of crucial intestinal cytoprotective defenses indicates a novel nutritional approach for the treatment of intestinal disease associated with altered redox homeostasis.

Lingua abstract: inglese

Pagine da: S248

Pagine a: S256


Molecular nutrition & food research Wiley-VCH-Verl..
Paese di pubblicazione: Germania
Lingua: inglese
ISSN: 1613-4133

Numero volume: 2

DOI: 10.1002/mnfr.201100295.

Referee: Sė: Internazionale

Stato della pubblicazione: Published version

Indicizzato da: PubMed [21954188]

Parole chiave:

  • Conjugated linoleic acid / Gluten toxicity / Nrf2-mediated defenses / Proteasome-acylpeptide hydrolase activity

Strutture CNR:


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