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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2009

Contributo in rivista

Tipo: Articolo in rivista

Titolo: The in vitro Nuclear Aggregates of Polyamines

Anno di pubblicazione: 2009

Formato: Elettronico Cartaceo

Autori: Di Luccia, Aldo; Picariello, Gianluca; Iacomino Giuseppe; Formisano, annarita; D'Agostino, Luciano

Affiliazioni autori: ISA-CNR

Autori CNR:

  • LUCIANO D'AGOSTINO
  • ALDO DI LUCCIA
  • ANNARITA FORMISANO
  • GIUSEPPE IACOMINO
  • GIANLUCA PICARIELLO

Abstract: Natural polyamines (putrescine, spermidine, and spermine) self-assemble in a simulated physiological environment (50 mm sodium phosphate buffer, pH 7.2), generating in vitro nuclear aggregates of polyamines (ivNAPs). These supramolecular compounds are similar in structure and molecular mass to naturally occurring cellular nuclear aggregates of polyamines, and they share the ability of NAPs to interact with and protect the genomic DNA against nuclease degradation. Three main ivNAP compounds were separated by gel permeation chromatography. Their elution was carried out with 50 mm sodium phosphate buffer supplemented with 150 mm NaCl. Freezing and thawing of selected chromatographic fractions obtained by GPC runs in which the mobile phase was sodium phosphate buffer not supplemented with NaCl yielded three different microcrystallites, specifically corresponding to the ivNAPs, all of which were able to bind DNA. In this study, we demonstrated that in vitro self-assembly of polyamines and phosphates is a spontaneous, reproducible and inexpensive event, and provided the indications for the production of the ivNAPs as a new tool for manipulating the genomic DNA machinery.

Lingua abstract: inglese

Pagine da: 2324

Pagine a: 2335

Rivista:

The FEBS journal Blackwell,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 1742-464X

Numero volume: 276

Referee: Sė: Internazionale

Parole chiave:

  • DNA conformation; DNA protection; molecular aggregates; polyamines; supramolecular chemistry

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