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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2008

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Transplastomic tobacco plants expressing a fatty acid desaturase gene exhibit altered fatty acid profiles and improved cold tolerance

Anno di pubblicazione: 2008

Formato: Cartaceo

Autori: Craig W., Lenzi P., Scotti N., De Palma M., Saggese P., Carbone V., McGrath Curran N., Magee A.M., Medgyesy P., Kavanagh T.A., Dix P.J., Grillo S., Cardi T.

Affiliazioni autori: CNR-IGV, Institute of Plant Genetics-Portici, Portici, NA, Italy CNR-ISA, Institute of Food Science, Avellino, AV, Italy Plant Cell Biology Laboratory, Biology Department, NUI-Maynooth, Maynooth, Ireland Plant Molecular Biology Laboratory, Smurfit Institute of Genetics, Trinity College, Lincoln Place Gate, Dublin 2, Ireland

Autori CNR:

  • VIRGINIA CARBONE
  • TEODORO CARDI
  • MONICA DE PALMA
  • MARIA STEFANIA GRILLO
  • PAOLA SAGGESE
  • NUNZIA SCOTTI

Lingua: inglese

Abstract: The possibility of altering the unsaturation level of fatty acids in plant lipids by genetic transformation has implications for the stress tolerance of higher plants as well as for their nutritional value and industrial utilisation. While the integration and expression of transgenes in the plastome has several potential advantages over nuclear transformation, very few attempts have been made to manipulate fatty acid biosynthesis using plastid transformation. We produced transplastomic tobacco plants that express a Delta(9) desaturase gene from either the wild potato species Solanum commersonii or the cyanobacterium Anacystis nidulans, using PEG-mediated DNA uptake by protoplasts. Incorporation of chloroplast antibiotic-insensitive point mutations in the transforming DNA was used to select transformants. The presence of the transcript and the Delta(9) desaturase protein in transplastomic plants was confirmed by northern and western blot analyses. In comparison with control plants, transplastomic plants showed altered fatty acid profiles and an increase in their unsaturation level both in leaves and seeds. The two transgenes produced comparable results. The results obtained demonstrate the feasibility of using plastid transformation to engineer lipid metabolic pathways in both vegetative and reproductive tissues and suggest an increase of cold tolerance in transplastomic plants showing altered leaf fatty acid profiles. This is the first example of transplastomic plants expressing an agronomically relevant gene produced with the "binding-type" vectors, which do not contain a heterologous marker gene. In fact, the transplastomic plants expressing the S. commersonii gene contain only plant-derived sequences, a clear attraction from a public acceptability perspective.

Pagine da: 769

Pagine a: 782

Rivista:

Transgenic research Chapman & Hall,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0962-8819

Numero volume: 17(5)

DOI: 10.1007/s11248-008-9164-9

Indicizzato da: ISI Web of Science (WOS) [000258719200002]

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