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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2008

Contributo in rivista

Tipo: Articolo in rivista

Titolo: 2-deoxy-d-ribose induces apoptosis by inhibiting the synthesis and increasing the efflux of glutathione

Anno di pubblicazione: 2008

Formato: Elettronico

Autori: Fico A.; Manganelli G.; Cigliano L.; Bergamo P.; Abrescia P.; Franceschi C.; Martini G.; Filosa S.

Affiliazioni autori: Institute of Genetics and Biophysics "Adriano Buzzati Traverso", CNR, Via Pietro Castellino 111, 80131 Napoli, Italy Dipartimento delle Scienze Biologiche, Universita' degli Studi di Napoli Federico II, Via Mezzocannone 8, 80134 Napoli, Italy Istituto di Scienze dell'Alimentazione - CNR, Via Roma 52 A/C, 83100 Avellino, Italy Department of Experimental Pathology, University of Bologna, via S. Giacomo 12, 40126 Bologna, Italy

Autori CNR:

  • PAOLO ABRESCIA
  • PAOLO BERGAMO
  • ANNALISA FICO
  • STEFANIA FILOSA
  • GENESIA MANGANELLI

Lingua: inglese

Abstract: Oxidative stress is caused by imbalance between the production of reactive oxygen species (ROS) and biological system ability to readily detoxify the reactive intermediates or repair the resulting damage. 2-deoxy-D-ribose (dRib) is known to induce apoptosis by provoking an oxidative stress by depleting glutathione (GSH). In this paper, we elucidate the mechanisms underlying GSH depletion in response to dRib treatment. We demonstrated that the observed GSH depletion is not only due to inhibition of synthesis, by inhibiting gamma-glutamyl-cysteine synthetase, but also due to its increased efflux, by the activity of multidrug resistance associated proteins transporters. We conclude that dRib interferes with GSH homeostasis and that likely cellular oxidative stress is a consequence of GSH depletion. Various GSH fates, such as direct oxidation, lack of synthesis or of storage, characterize different kinds of oxidative stress. In the light of our observations we conclude that dRib does not induce GSH oxidation but interferes with GSH synthesis and storage. Lack of GSH allows accumulation of ROS and cells, disarmed against oxidative insults, undergo apoptosis.

Lingua altro abstract: inglese

Pagine da: 211

Pagine a: 217

Rivista:

Free radical biology & medicine Pergamon,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0891-5849

Numero volume: 45

Numero fascicolo: 2

DOI: 10.1016/j.freeradbiomed.2008.04.017

Referee: Ś: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000257089100014]

Parole chiave:

  • oxidative stress
  • dRib
  • G6PD
  • GSH
  • embryonic stem cells

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