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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2010

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Computerized sperm motility analysis in toxicity bioassays: A new approach to pore water quality assessment

Anno di pubblicazione: 2010

Formato: Elettronico Cartaceo

Autori: Fabbrocini A; Di Stasio M; D'Adamo R

Affiliazioni autori: Consiglio Nazionale delle Ricerche--Istituto di Scienze Marine, UOS Lesina, via Pola 4, 71010 Lesina (FG), Italy Consiglio Nazionale delle Ricerche--Istituto di Scienze dell'Alimentazione, via Roma 52, 83100 Avellino, Italy

Autori CNR:


Lingua: inglese

Abstract: The aim of this study was to test the sensitivity of computerized sperm motility analysis in the sea urchin Paracentrotus lividus as the endpoint in toxicity bioassays. The tested matrices were pore water samples collected in an agriculture-impacted Mediterranean lagoon, Lake Varano (Italy). Two standardized bioassays were also conducted as controls, the P. lividus spermiotoxicity test and the Vibrio fischeri (Microtoxs) test. VCL (curvilinear velocity), VSL (straight line velocity), VAP (average path velocity), and the percentage of rapid spermatozoa recorded by the Sperm Class Analyzers system showed high sensitivity and discrimination ability, to a degree comparable with the larval development endpoint of the spermiotoxicity test. The test evaluated in this study requires small volumes of matrices, involves minimal sample manipulation, and can easily be extended to many other bioindicator species. It may therefore be considered a promising ''quick response tool'' following hazardous events that may adversely affect an aquatic ecosystem.

Lingua abstract: inglese

Pagine da: 1588

Pagine a: 1595

Pagine totali: 8


Ecotoxicology and environmental safety Elsevier [etc.]
Paese di pubblicazione: Paesi Bassi
Lingua: inglese
ISSN: 0147-6513

Numero volume: 73

DOI: 10.1016/j.ecoenv.2010.05.003

Referee: Ś: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000282792300014]

Parole chiave:

  • Toxicity bioassays
  • Sea urchin
  • Computerized sperm motility analysis
  • Embryonic development
  • Microtox

URL: http://scienceserver.cilea.it/pdflinks/12032615471606842.pdf

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