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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2003

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Cytosine-block telomeric type DNA-binding activity of hnRNP proteins from human cell lines

Anno di pubblicazione: 2003

Autori: Bandiera A., Tell G., Marsich E., Scaloni A., Pocsfalvi G., Akindahunsi A.A., Cesaratto L. and Manzini G.

Autori CNR:


Abstract: Following the observation of the presence in mammalian nuclear extracts of a DNA binding activity quite specific for the single-stranded C-rich telomeric motif, we have isolated from the K562 human cell line by affinity chromatography and identified by mass spectrometry a number of proteins able to bind to this sequence. All of them belong to different heterogeneous nuclear ribonucleoprotein subgroups (hnRNP). Whereas many of them, namely hnRNP K, two isoforms of hnRNP I, and the factor JKTBP, appear to bind to this sequence with limited specificity after isolation, an isoform of hnRNP D (alias AUF1) and particularly hnRNP E1 (alias PCBP-1) show a remarkable specificity for the (CCCTAA)n repeated motif. Both have been obtained also as recombinant proteins expressed in Escherichia coli and have been shown to retain their binding specificity toward the C-block repeated sequence. In the light of the current knowledge about these proteins, their possible involvement in telomere functioning is discussed

Pagine da: 305

Pagine a: 314


Archives of biochemistry and biophysics Elsevier Inc.
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0003-9861

Numero volume: 409

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