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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2006

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Study of the three-way interaction between Trichoderma atroviride, plant and fungal pathogens by using a proteomic approach

Anno di pubblicazione: 2006

Formato: Elettronico Cartaceo

Autori: Marra R., Ambrosino P., Vinale F., Woo S.L., Ciliento R., Lanzuise S., Ferraioli S., Soriente I., Gigante S., Turrà D., Carbone V., Lorito M., Ruocco M., Scala F., Fogliano V.

Affiliazioni autori: Dipartimento di Arboricoltura, Botanica e Patologia Vegetale, Università degli Studi di Napoli Federico II, 80055 Portici (NA), Italy Centro di Spettrometria di Massa Proteomica e Biomolecolare, Istituto di Scienze dell’Alimentazione del CNR, Avellino, Italy Istituto CNR per la Protezione delle Piante (IPP),Portici (NA), Italy Università degli Studi di Napoli Federico II, Portici (NA), Italy

Autori CNR:

  • VIRGINIA CARBONE
  • MATTEO LORITO
  • MICHELINA RUOCCO
  • IDA SORIENTE

Lingua: inglese

Abstract: The main molecular factors involved in the complex interactions occurring between plants (bean), two different fungal pathogens (Botrytis cinerea, Rhizoctonia solani) and an antagonistic strain of the genus Trichoderma were investigated. Two-dimensional (2-D) electrophoresis was used to analyze separately collected proteomes from each single, two- or three-partner interaction (i.e., plant, pathogenic and antagonistic fungus alone and in all possible combinations). Differential proteins were subjected to mass spectrometry and in silico analysis to search for homologies with known proteins. In the plant proteome, specific pathogenesis-related proteins and other disease-related factors (i.e., potential resistance genes) seem to be associated with the interaction with either one of the two pathogens and/or T. atroviride. This finding is in agreement with the demonstrated ability of Trichoderma spp. to induce systemic resistance against various microbial pathogens. On the other side, many differential proteins obtained from the T. atroviride interaction proteome showed interesting homologies with a fungal hydrophobin, ABC transporters, etc. Virulence factors, like cyclophilins, were up-regulated in the pathogen proteome during the interaction with the plant alone or with the antagonist too. We isolated and confidently identified a large number of protein factors associated to the multi-player interactions examined.

Lingua abstract: inglese medio (1100-1500)

Pagine da: 307

Pagine a: 321

Rivista:

Current genetics Springer
Paese di pubblicazione: Germania
Lingua: inglese
ISSN: 0172-8083

Numero volume: 50

Referee: Sì: Internazionale

Parole chiave:

  • Trichoderma
  • Proteomics
  • Plant
  • Fungal pathogens
  • Differential proteins

Strutture CNR:

Moduli:

Allegati: marra et al 2006 (application/pdf)

 
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