Home |  English version |  Mappa |  Commenti |  Sondaggio |  Staff |  Contattaci Cerca nel sito  
Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2005

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Antioxidant and cytotoxic properties of lyophilized beer extracts on HL-60 cell line.

Anno di pubblicazione: 2005

Formato: Elettronico Cartaceo

Autori: Tedesco Idolo; Nappo Annunziata; Petitto Fabio; Iacomino Giuseppe; Nazzaro Filomena; Palumbo Rosanna; Russo Gian Luigi

Affiliazioni autori: Istituto Scienze dell'Alimentazione

Autori CNR:

  • GIUSEPPE IACOMINO
  • ANNUNZIATA NAPPO
  • FILOMENA NAZZARO
  • ROSANNA PALUMBO
  • GIAN LUIGI RUSSO
  • IDOLO TEDESCO

Abstract: An impressive number of studies have suggested that red wine can be considered the protective beverage of choice against chronic and degenerative pathologies. Only few and controversial data are available on a potential, similar role for beer, which represents a more cost-effective, safe, and widely available beverage. Starting from the evidence that many antioxidant compounds present in red wine are also present at similar or even higher concentrations in beers, we first screened 48 commercially available beers and selected one (Mrt-HP) with very high polyphenol concentration and antioxidant activity estimated by ferric reducing antioxidant power. We demonstrated that a lyophilized preparation of Mrt-HP beer was cytotoxic with respect to a beer with low polyphenolic content (Trt-LP) when assayed on HL-60 human leukemia cell line. We measured a 60% decrease in cell viability at a polyphenol concentration of 250 microM quercetin equivalents. We also demonstrated that Mrt-HP cytotoxicity was not an artifact due to cell growth conditions because addition of Mrt-HP extracts to cell medium generated peroxide levels indistinguishable from controls. By means of cytofluorimetric analysis of pre-G1 population and caspase 3 activation, we demonstrated that Mrt-HP extracts activated apoptosis in HL-60 cell line. Finally, we found that the concentration of quercetin, resveratrol, and gallic acid in Mrt-HP was 10, 4.6, and 4.6-fold higher, respectively, than in Trt-LP, suggesting that the presence of these molecules might be responsible for the observed cytotoxicity. These data, together with the low in vivo beer toxicity reported in the literature, suggest a possible chemopreventive role for this beverage that requires further studies in animal models.

Lingua abstract: inglese

Pagine da: 74

Pagine a: 83

Rivista:

Nutrition and cancer Routledge, Taylor & Francis Group
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0163-5581

Numero volume: 52

DOI: 10.1207/s15327914nc5201_10

Referee: Sì: Internazionale

Indicizzato da:

  • PubMed [16091007]
  • ISI Web of Science (WOS) [000231482400010]

Parole chiave:

  • lyophilized beer extracts
  • Antioxidant property
  • cytotoxic property

Strutture CNR:

Moduli:

 
Torna indietro Richiedi modifiche Invia per email Stampa
Home Il CNR  |  I servizi News |   Eventi | Istituti |  Focus