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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2009

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Structure and stability of a rat odorant-binding protein. Another brick in the wall

Anno di pubblicazione: 2009

Formato: Elettronico

Autori: Scirč A.; Marabotti A.; Staiano M.; Briand L.; Varriale A.; Bertoli E.; Tanfani F.; D'Auria S.

Affiliazioni autori: Department of Biochemistry, Biology, and Genetics, Universita? Politecnica delle Marche, Ancona, Italy, Institute of Food Sciences, CNR, Avellino, Italy, Laboratory for Molecular Sensing, IBP, CNR, Naples, Italy, and UMR FLAVIC INRA-ENESAD - University of Bourgogne, 17 rue Sully, BP 86510 21065 Dijon, Cedex, France

Autori CNR:

  • SABATO D'AURIA
  • ANNA MARABOTTI
  • MARIA STAIANO
  • ANTONIO VARRIALE

Lingua: inglese

Abstract: The effect of temperature on the structure of the rat odorant-binding protein was investigated by spectroscopic and in silico methodologies. In particular, in this work, we examined the structural features of the rat OBP-1F by Fourier-transform infrared spectroscopy and molecular dynamics investigations. The obtained spectroscopic results were analyzed using the following three different methods based on the unexchanged amide hydrogens of the protein sample: (1) the analysis of difference spectra; (2) the generalized 2D-IR correlation spectroscopy; (3) the phase diagram method. The three methods indicated that at high temperatures the rOBP-1F structure undergoes a relaxation process involving the protein tertiary organization before undergoing the denaturation and aggregation processes, suggesting the presence of an intermediate state such as a molten globule-like state. Importantly, the proposed analyses represent a general approach that could be applied to the study of protein stability.

Lingua abstract: inglese

Pagine da: 4005

Pagine a: 4013

Rivista:

Journal of proteome research American Chemical Society,
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 1535-3893

Numero volume: 8

Numero fascicolo: 8

DOI: 10.1021/pr900346z

Referee: Sė: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000268661800024]

Parole chiave:

  • Odorant-binding protein
  • Fourier transform infrared spectroscopy
  • Lipocalin

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