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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2007

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Stability and dynamics of the porcine odorant-binding protein

Anno di pubblicazione: 2007

Formato: Elettronico

Autori: Staiano M.; D'Auria S.; Varriale A.; Rossi M.; Marabotti A.; Fini C.; Stepanenko O.V.; Kuznetsova I.M.; Turoverov K.K.

Affiliazioni autori: Institute of Protein Biochemistry, CNR, Naples, Italy, Institute of Food Sciences, CNR, AVellino, Italy, University of Perugia, Italy, and Institute of Cytology, RAS, St. Petersburg, Russia

Autori CNR:

  • SABATO D'AURIA
  • ANNA MARABOTTI
  • MOSč ROSSI
  • MARIA STAIANO
  • ANTONIO VARRIALE

Lingua: inglese

Abstract: The denaturation process of porcine odorant-binding protein (pOBP) was studied by intrinsic fluorescence analysis and far- and near-UV circular dichroism measurements. Our results showed that a reversible one-step process described the denaturation by GdnHCl. The midpoint of the transition, that is, the point where the free energies of protein in the native and unfolded states are equal, corresponds to 2.3 M GdnHCl. The difference in free energy between native and unfolded states of pOBP is -5.95 kcal/mol in the absence of GdnHCl, indicating that the protein molecule is very stable to the denaturing action of GdnHCl. A 15% increase in fluorescence intensity accompanied by a 25% decrease of fluorescence decay lifetime recorded in the range of 0.0-1.4 M GdnHCl was explained by the destruction of the complex between Trp 16 and the positively charged atom NZ of Lys 120, localized over the center of the Trp 16 indole ring, with concurrent formation of complex between Trp 16 and bound water molecules also located in its close vicinity.

Lingua abstract: inglese

Pagine da: 11120

Pagine a: 11127

Rivista:

Biochemistry American Chemical Society.
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0006-2960

Numero volume: 46

Numero fascicolo: 39

DOI: 10.1021/bi7008129

Referee: Sė: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000249726500011]

Parole chiave:

  • TRYPTOPHAN FLUORESCENCE
  • MOLECULAR-DYNAMICS
  • DEPENDENCE
  • LIPOCALINS
  • MECHANISM

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