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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2011

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Identification of proteins associated with ligand-activated estrogen receptor in human breast cancer cell nuclei by tandem affinity purification and nano LC-MS/MS.

Anno di pubblicazione: 2011

Formato: Elettronico Cartaceo

Autori: Tarallo R, Bamundo A, Nassa G, Nola E, Paris O, Ambrosino C, Facchiano A, Baumann M, Nyman TA, Weisz A

Affiliazioni autori: 1 Department of General Pathology, Second University of Naples, Napoli, Italy 2 Department of Biological and Environmental Sciences, University of Sannio, Benevento, Italy 3 Institute of Food Science, National Research Council, Avellino, Italy 4 Institute of Biomedicine (Physiology), Biomedicum Helsinki, University of Helsinki, Helsinki, Finland 5 Protein Chemistry Unit, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland 6 Protein Chemistry Research Group, Institute of Biotechnology, University of Helsinki, Helsinki, Finland 7 Molecular Medicine Laboratory, Faculty of Medicine and Surgery, University of Salerno, Baronissi, Italy

Autori CNR:

  • ANGELO FACCHIANO

Lingua: inglese

Abstract: Estrogen receptor a (ER-a) is a key mediator of estrogen actions in breast cancer (BC) cells. Understanding the effects of ligand-activated ER-a in target cells requires identification of the molecular partners acting in concert with this nuclear receptor to transduce the hormonal signal. We applied tandem affinity purification (TAP), glycerol gradient centrifugation and MS analysis to isolate and identify proteins interacting with ligand-activated ER-a in MCF-7 cell nuclei. This led to the identification of 264 ER-associated proteins, whose functions highlight the hinge role of ER-a in the coordination of multiple hormone-regulated nuclear processes in BC cells.

Lingua abstract: inglese

Pagine da: 172

Pagine a: 179

Rivista:

Proteomics Wiley-VCH-Verl.
Paese di pubblicazione: Germania
Lingua: inglese
ISSN: 1615-9853

Numero volume: 11

Stato della pubblicazione: Published version

Parole chiave:

  • Breast cancer / Cell biology / Estrogen receptor / Interactome / Nuclear proteins / Tandem affinity purification

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