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Contributo in rivista
Tipo: Articolo in rivista
Titolo: Differential alterations of the concentrations of endocannabinoids and related lipids in the subcutaneous adipose tissue of obese diabetic patients.
Anno di pubblicazione: 2010
Formato: Elettronico Cartaceo
Autori: Annuzzi G; Piscitelli F; Di Marino L; Patti L; Giacco R; Costabile G; Bozzetto L; Riccardi G; Verde R; Petrosino S; Rivellese AA; Di Marzo V.
Affiliazioni autori: Department of Clinical and Experimental Medicine, Federico II University, Naples, Italy Endocannabinoid Research Group, Institute of Biomolecular Chemistry, CNR, Pozzuoli Naples, Italy National Research Council, Avellino, Italy
Abstract: Abstract: Background: The endocannabinoids, anandamide and 2-AG, are produced by adipocytes, where they stimulate lipogenesis via cannabinoid CB(1) receptors and are under the negative control of leptin and insulin. Endocannabinoid levels are elevated in the blood of obese individuals and nonobese type 2 diabetes patients. To date, no study has evaluated endocannabinoid levels in subcutaneous adipose tissue (SAT) of subjects with both obesity and type 2 diabetes (OBT2D), characterised by similar adiposity and whole body insulin resistance and lower plasma leptin levels as compared to non-diabetic obese subjects (OB). Design and Methods: The levels of anandamide and 2-AG, and of the anandamide-related PPAR alpha ligands, oleoylethanolamide (OEA) and palmitoylethanolamide (PEA), in the SAT obtained by abdominal needle biopsy in 10 OBT2D, 11 OB, and 8 non-diabetic normal-weight (NW) subjects, were measured by liquid chromatography-mass spectrometry. All subjects underwent a hyperinsulinaemic euglycaemic clamp. Results: As compared to NW, anandamide, OEA and PEA levels in the SAT were 2-4.4-fold elevated (p < 0.05), and 2-AG levels 2.3-fold reduced (p <.05), in OBT2D but not in OB subjects. Anandamide, OEA and PEA correlated positively (p <.05) with SAT leptin mRNA and free fatty acid during hyperinsulinaemic clamp, and negatively with SAT LPL activity and plasma HDL-cholesterol, which were all specifically altered in OBT2D subjects. Conclusions: The observed alterations emphasize, for the first time in humans, the potential different role and regulation of adipose tissue anandamide (and its congeners) and 2-AG in obesity and type 2 diabetes.
Lingua abstract: inglese
Pagine da: 43
Pagine totali: 8
Lipids in health and disease
Numero volume: 9
Referee: Sì: Internazionale
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