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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in volume anno 2001

Contributo in volume

Tipo: Contributo in volume

Titolo: Detection of plasmin-mediated beta-casein hydrolysis in milk and cheese through immunochemical techniques

Anno di pubblicazione: 2001

Formato: Cartaceo

: Capitolo

Autori: Pizzano Rosa; Nicolai Maria Adalgisa; Chianese Lina; Addeo Francesco

Affiliazioni autori: Pizzano Rosa: Istituto di scienza dell'alimentazione; Nicolai Maria Adalgisa, Chianese Lina, Addeo Francesco: Dipartimento di Scienza degli Alimenti - Università degli Studi di Napoli Federico II

Autori CNR:

  • ROSA PIZZANO

Lingua: inglese

Sintesi: In this article recent advances in development of selective immunochemical reagents to monitor plasmin-mediated beta-casein hydrolysis are reported and briefly discussed. The attention was mainly focused on antipeptide antibodies specifically recognizing significant peptides derived from beta-casein plasmin hydrolysis in milk and cheese. Analytical potentialities of the polyclonal antibodies raised against native or synthetic peptides were evaluated and possible applications of the antipeptide antibodies preparations to the quality control of dairy products were suggested. Progression of plasmin-mediated hydrolysis of beta-casein occurring in Parmigiano Reggiano cheese during ripening was easily followed by immunoblotting using antipeptide antibodies raised against the five amino acid residue peptide reproducing the N terminus of gamma2- casein. The release of the 1-28 phosphopeptide from beta-casein was monitored in maturing cheese by competitive ELISA using antipeptide antibodies raised against RP-HPLC purified 1-28 phosphopeptide from bovine beta-casein. Finally, a preparation of antipeptide antibodies, raised against a synthetic peptide carrying a properly chosen bovine beta-casein sequence, was able to reveal selectively bovine and water buffalo beta-casein and their derived gamma2-and gamma3-caseins but not the ovine and caprine counterparts, so that it is a suitable reagent for ELISA detection of bovine casein in adultered ovine and caprine cheese samples.

Lingua sintesi: eng

Pagine da: 191

Pagine a: 199

Pagine totali: 9

Titolo del volume: Recent Research Developments in Agricultural and Food Chemistry

N. volume della serie/collana: 5

Curatore/i del volume: Pandalai S. G. ed

ISBN: 9788177360752

Editore: Research Signpost, Trivandrum (IND)

Referee: Sì: Internazionale

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